Metabolomic characterization of human hippocampus from drug-resistant epilepsy with mesial temporal seizure

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buir.contributor.authorÇiçek, A. Ercüment
dc.citation.epage616en_US
dc.citation.issueNumber3en_US
dc.citation.spage607en_US
dc.citation.volumeNumber59en_US
dc.contributor.authorDetour, J.en_US
dc.contributor.authorBund, C.en_US
dc.contributor.authorBehr, C.en_US
dc.contributor.authorCebula, H.en_US
dc.contributor.authorÇiçek, A. Ercümenten_US
dc.contributor.authorValenti-Hirsch, Maria-Paolaen_US
dc.contributor.authorLannes, B.en_US
dc.contributor.authorLhermitte, B.en_US
dc.contributor.authorNehlig, A.en_US
dc.contributor.authorKehrli, P.en_US
dc.contributor.authorProust, F.en_US
dc.contributor.authorHirsch, E.en_US
dc.contributor.authorNamer, Izzie-Jacquesen_US
dc.date.accessioned2019-02-12T08:42:53Z
dc.date.available2019-02-12T08:42:53Z
dc.date.issued2018-03en_US
dc.departmentDepartment of Computer Engineeringen_US
dc.description.abstractWithin a complex systems biology perspective, we wished to assess whether hippocampi with established neuropathological features have distinct metabolome. Apparently normal hippocampi with no signs of sclerosis (noHS), were compared to hippocampal sclerosis (HS) type 1 (HS1) and/or type 2 (HS2). Hippocampus metabolome from patients with epilepsy-associated neuroepithelial tumors (EANTs), namely, gangliogliomas (GGs) and dysembryoplastic neuroepithelial tumors (DNTs), was also compared to noHS epileptiform tissue. Methods: All patients underwent standardized temporal lobectomy. We applied 1H high-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) spectroscopy to 48 resected human hippocampi. NMR spectra allowed quantification of 21 metabolites. Data were analyzed using multivariate analysis based on mutual information. Results: Clear distinct metabolomic profiles were observed between all studied groups. Sixteen and 18 expected metabolite levels out of 21 were significantly different for HS1 and HS2, respectively, when compared to noHS. Distinct concentration variations for glutamine, glutamate, and N-acetylaspartate (NAA) were observed between HS1 and HS2. Hippocampi from GG and DNT patients showed 7 and 11 significant differences in metabolite concentrations when compared to the same group, respectively. GG and DNT had a clear distinct metabolomic profile, notably regarding choline compounds, glutamine, glutamate, aspartate, and taurine. Lactate and acetate underwent similar variations in both groups. Significance: HRMAS NMR metabolomic analysis was able to disentangle metabolic profiles between HS, noHS, and epileptic hippocampi associated with EANT. HRMAS NMR metabolomic analysis may contribute to a better identification of abnormal biochemical processes and neuropathogenic combinations underlying mesial temporal lobe epilepsy.en_US
dc.embargo.release2019-03-04en_US
dc.identifier.doi10.1111/epi.14000en_US
dc.identifier.eissn1528-1167
dc.identifier.issn0013-9580
dc.identifier.urihttp://hdl.handle.net/11693/49304
dc.language.isoEnglishen_US
dc.publisherWiley-Blackwell Publishingen_US
dc.relation.isversionofhttps://doi.org/10.1111/epi.14000en_US
dc.source.titleEpilepsiaen_US
dc.subjectHippocampal sclerosisen_US
dc.subjectHRMAS NMRen_US
dc.subjectLong-term epilepsy-associated tumoren_US
dc.subjectMesial temporal lobe epilepsyen_US
dc.subjectMetabolomicsen_US
dc.titleMetabolomic characterization of human hippocampus from drug-resistant epilepsy with mesial temporal seizureen_US
dc.typeArticleen_US

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