Autologous anti-SOX2 antibody responses reflect intensity but not frequency of antigen expression in small cell lung cancer

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dc.citation.issueNumber1en_US
dc.citation.volumeNumber14en_US
dc.contributor.authorAtakan, S.en_US
dc.contributor.authorBayiz, H.en_US
dc.contributor.authorSak, S.en_US
dc.contributor.authorPoyraz, A.en_US
dc.contributor.authorVural, B.en_US
dc.contributor.authorYildirim, A. S.en_US
dc.contributor.authorDemirag, F.en_US
dc.contributor.authorGure, A. O.en_US
dc.date.accessioned2016-02-08T10:52:25Z
dc.date.available2016-02-08T10:52:25Z
dc.date.issued2014en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: Anti-SOX2 antibody responses are observed in about 10 to 20% of small cell lung cancer (SCLC) patients. The aim of this study was to determine whether such responses reflect a particular pattern of SOX2 protein expression in the tumor and whether this pattern associates with clinical outcome. Methods. Paraffin embedded tumor tissues, obtained from SCLC patients who had no evidence of paraneoplastic autoimmune degeneration, were evaluated for SOX2 expression by immunohistochemistry for both intensity and extent of staining. Sera from the same patients were tested for autologous antibodies against recombinant SOX2 by enzyme-linked immunosorbent assay (ELISA). Correlates between overall survival and various clinical parameters including SOX2 staining and serology were determined. Results: SOX2 protein expression was observed in tumor tissue in 89% of patients. Seventeen patients (29%) were seropositive for SOX2 antibodies and, in contrast to SOX2 staining, the presence of antibody correlated with limited disease stage (p = 0.05). SOX2 seropositivity showed a significant association with the intensity of SOX2 staining in the tumor (p = 0.02) but not with the frequency of SOX2 expressing cells. Conclusion: Anti-SOX2 antibodies associate with better prognosis (limited stage disease) while SOX2 protein expression does not; similar to reports from some earlier studies. Our data provides an explanation for this seemingly contrasting data for the first time as SOX2 antibodies can be observed in patients whose tumors contain relatively few but strongly staining cells, thus supporting the possible presence of active immune-surveillance and immune-editing targeting SOX2 protein in this tumor type. © 2014 Atakan et al.; licensee BioMed Central Ltd.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:52:25Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1186/1472-6890-14-24en_US
dc.identifier.issn1472-6890
dc.identifier.urihttp://hdl.handle.net/11693/25925
dc.language.isoEnglishen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/1472-6890-14-24en_US
dc.source.titleBMC Clinical Pathologyen_US
dc.subjectCancer stem cellsen_US
dc.titleAutologous anti-SOX2 antibody responses reflect intensity but not frequency of antigen expression in small cell lung canceren_US
dc.typeArticleen_US

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