Detection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beacons

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dc.citation.epage96en_US
dc.citation.issueNumber1en_US
dc.citation.spage90en_US
dc.citation.volumeNumber352en_US
dc.contributor.authorKam, Y.en_US
dc.contributor.authorRubinstein, A.en_US
dc.contributor.authorNaik, S.en_US
dc.contributor.authorDjavsarov, I.en_US
dc.contributor.authorHalle, D.en_US
dc.contributor.authorAriel, I.en_US
dc.contributor.authorGure, A. O.en_US
dc.contributor.authorStojadinovic, A.en_US
dc.contributor.authorPan, H. G.en_US
dc.contributor.authorTsivin, V.en_US
dc.contributor.authorNissan, A.en_US
dc.contributor.authorYavin, E.en_US
dc.date.accessioned2019-02-10T21:00:40Z
dc.date.available2019-02-10T21:00:40Z
dc.date.issued2014-09-28en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractAlthough the function and mechanism of action of long non-coding RNAs (lncRNA) is still not completely known, studies have shown their potential role in the control of gene expression and regulation, in cellular proliferation and invasiveness at the transcriptional level via multiple mechanisms. Recently, colon cancer associated transcript 1 (CCAT1) lncRNA was found to be expressed in colorectal cancer (CRC) tumors but not in normal tissue. This study aimed to study the ability of a CCAT1-specific peptide nucleic acid (PNA) based molecular beacons (TO-PNA-MB) to serve as a diagnostic probe for in vitro, ex vivo, and in situ (human colon biopsies) detection of CRC. The data showed enhanced fluorescence upon in vitro hybridization to RNA extracted from CCAT1 expressing cells (HT-29, SW-480) compared to control cells (SK-Mel-2). Uptake of TO-PNA-MBs into cells was achieved by covalently attaching cell penetrating peptides (CPPs) to the TO-PNA-MB probes. In situ hybridization of selected TO-PNA-MB in human CRC specimens was shown to detect CCAT1 expression in all (4/4) subjects with pre-cancerous adenomas, and in all (8/8) patients with invasive adenocarcinoma (penetrating the bowel wall) tumors. The results showed that CCAT1 TO-PNA-MB is a powerful diagnostic tool for the specific identification of CRC, suggesting that with the aid of an appropriate pharmaceutical vehicle, real time in vivo imaging is feasible. TO-PNA-MB may enable identifying occult metastatic disease during surgery, or differentiating in real time in vivo imaging, between benign and malignant lesions.en_US
dc.description.provenanceSubmitted by M. Arif Kart (arif.kart@bilkent.edu.tr) on 2019-02-10T21:00:40Z No. of bitstreams: 1 Detection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beacons.pdf: 3663625 bytes, checksum: 462a23d06f4348dedfe9d454546e0e37 (MD5)en
dc.description.provenanceMade available in DSpace on 2019-02-10T21:00:40Z (GMT). No. of bitstreams: 1 Detection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beacons.pdf: 3663625 bytes, checksum: 462a23d06f4348dedfe9d454546e0e37 (MD5) Previous issue date: 2014-09-28en
dc.identifier.doi10.1016/j.canlet.2013.02.014en_US
dc.identifier.issn0304-3835
dc.identifier.urihttp://hdl.handle.net/11693/49186
dc.language.isoEnglishen_US
dc.publisherElsevier Ireland Ltd.en_US
dc.relation.isversionofhttps://doi.org/10.1016/j.canlet.2013.02.014en_US
dc.source.titleCancer Lettersen_US
dc.subjectColorectal canceren_US
dc.subjectMolecular beaconen_US
dc.subjectPeptide nucleic aciden_US
dc.subjectLong non-coding RNAen_US
dc.subjectImagingen_US
dc.titleDetection of a long non-coding RNA (CCAT1) in living cells and human adenocarcinoma of colon tissues using FIT–PNA molecular beaconsen_US
dc.typeArticleen_US

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