Smoking and nicotine alter UGT1A expression

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buir.advisorKonu, Özlen
dc.contributor.authorÖlmezer, Gizem
dc.date.accessioned2016-01-08T18:23:51Z
dc.date.available2016-01-08T18:23:51Z
dc.date.issued2011
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionAnkara : The Department of Molecular Biology and Genetics and the Graduate School of Engineering and Science of Bilkent University, 2011.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2011.en_US
dc.descriptionIncludes bibliographical references leaves 93-110.en_US
dc.description.abstractThe expression and activity of enzymes taking role in drug metabolism are important as in the case of phase II glucuronidation enzymes; namely UDPglucuronosyltransferases (UGTs). Previously, it has been identified that smoking upregulates the expression of UGT enzymes in oral mucosa. We asked whether smoking induces UGT1A expression in other tissues and re-analyzed publically available datasets run with samples from smokers and non-smokers. It was observed that UGT1A enzymes were overexpressed in several types of epithelial cells of smokers. 30% of nicotine metabolism is performed by UGT enzymes; however, whether UGT1A expression is modulated by nicotine, the addictive component of tobacco smoke, is not known. For this purpose, the expression levels of UGT1A isoforms were measured using Real-Time PCR in nicotine treated SW620 colorectal cancer cells. Our findings showed that nicotine’s effect on UGT1A expression was isoform specific; and the magnitude of modulation differed among isoforms. Furthermore, the upregulation of UGT1A enzymes could only be observed in serum-deprived SW620 cells. In summary, nicotine metabolism enzymes are regulated by both smoking in vivo and nicotine in vitro. Nevertheless, enhanced xenobiotic metabolism may result in chemoresistance, which is undesirable for cancer patients. Therefore, before drug therapy cancer patients might be analyzed in terms of their smoking status and UGT1A expression patterns.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityÖlmezer, Gizemen_US
dc.format.extentxv, 110 leaves, illustrationsen_US
dc.identifier.urihttp://hdl.handle.net/11693/15737
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNicotineen_US
dc.subjectSmokingen_US
dc.subjectUDP-glucuronosyltransferases (UGTs)en_US
dc.subjectSerum Starvationen_US
dc.subjectColon Canceren_US
dc.subject.lccQP801.N48 O56 2011en_US
dc.subject.lcshNicotine--Metabolism.en_US
dc.subject.lcshNicotine--Physiological effects.en_US
dc.subject.lcshColon (Anatomy)--Cancer.en_US
dc.subject.lcshEnzymes.en_US
dc.titleSmoking and nicotine alter UGT1A expressionen_US
dc.typeThesisen_US

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Dept. of Molecular Biology and Genetics - Master's degree