Immobilization of laccase on itaconic acid grafted and Cu ( II ) ion chelated chitosan membrane for bioremediation of hazardous materials

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dc.citation.epage539en_US
dc.citation.issueNumber4en_US
dc.citation.spage530en_US
dc.citation.volumeNumber87en_US
dc.contributor.authorBayramoglu, G.en_US
dc.contributor.authorGursel, I.en_US
dc.contributor.authorYilmaz, M.en_US
dc.contributor.authorArica, M. Y.en_US
dc.date.accessioned2016-02-08T09:47:27Z
dc.date.available2016-02-08T09:47:27Z
dc.date.issued2012en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: Chitosan membranes were formed through a phase inversion technique and then cross-linked with epichlorohydrin (CHX). Heterogeneous graft copolymerization of itaconic acid (IA) onto membrane was carried out with different monomer concentrations (CHX-g-p(IA)). The membrane properties such as equilibrium swelling ratio, porosity, and contact angle were measured, together with analysis by scanning electron microscopy (SEM), energy dispersive analysis of X-rays (EDAX), atomic force microscopy (AFM), and Fourier transform infrared (FTIR) spectroscopy. Results: The Cu(II) ion incorporated membranes (i.e. CHX-g-p(IA)-Cu(II)) were used for reversible immobilization of laccase using CHX-g-p(IA) membrane as a control system. Maximum laccase adsorption capacities of the CHX-g-p(IA) and CHX-g-p(IA)-Cu(II) membranes (with 9.7% grafting yield) were found to be 6.3 and 17.6 mg mL -1 membrane at pH 4.0 and 6.0, respectively. The K m value for immobilized laccase on CHX-g-p(IA)-Cu(II) (4.16 × 10 -2 mmol L -1) was 2.11-fold higher than that of free enzyme (1.97 × 10 -2 mmol L -1). Finally, the immobilized laccase was used in a batch system for degradation of three different dyes (Reactive Black 5, RB5; Cibacron Blue F3GA, CB; and Methyl Orange, MO). The immobilized laccase on CHX-g-p(IA)-Cu(II) membrane was more effective for removal of MO dye than removal of CB and RB5 dyes. CONCLUSION: Flexibility of the enzyme immobilized grafted polymer chains is expected to provide easy reaction conditions without diffusion limitation for substrate dye molecules and their products. The support described, prepared from green chemicals, can be used for the immobilization of industrially important enzymes. © 2012 Society of Chemical Industry.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:47:27Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2012en
dc.identifier.doi10.1002/jctb.2743en_US
dc.identifier.eissn1097-4660
dc.identifier.issn0268-2575
dc.identifier.urihttp://hdl.handle.net/11693/21518
dc.language.isoEnglishen_US
dc.publisherWileyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/jctb.2743en_US
dc.source.titleJournal of Chemical Technology and Biotechnologyen_US
dc.subjectChitosanen_US
dc.subjectItaconic aciden_US
dc.subjectGraft copolymerizationen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectLaccaseen_US
dc.subjectDye degradationen_US
dc.titleImmobilization of laccase on itaconic acid grafted and Cu ( II ) ion chelated chitosan membrane for bioremediation of hazardous materialsen_US
dc.typeArticleen_US

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