A monoclonal antibody against DNA binding helix of p53 protein
dc.citation.epage | 1401 | en_US |
dc.citation.issueNumber | 11 | en_US |
dc.citation.spage | 1398 | en_US |
dc.citation.volumeNumber | 20 | en_US |
dc.contributor.author | Yolcu, E. | en_US |
dc.contributor.author | Sayan, B. S. | en_US |
dc.contributor.author | Yağci, T. | en_US |
dc.contributor.author | Cetin Atalay, R. | en_US |
dc.contributor.author | Soussi, T. | en_US |
dc.contributor.author | Yurdusev, N. | en_US |
dc.contributor.author | Ozturk, M. | en_US |
dc.date.accessioned | 2016-02-08T10:35:40Z | |
dc.date.available | 2016-02-08T10:35:40Z | |
dc.date.issued | 2001 | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.description.abstract | Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins. | en_US |
dc.description.provenance | Made available in DSpace on 2016-02-08T10:35:40Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2001 | en |
dc.identifier.doi | 10.1038/sj.onc.1204240 | en_US |
dc.identifier.issn | 0950-9232 | |
dc.identifier.uri | http://hdl.handle.net/11693/24881 | |
dc.language.iso | English | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/sj.onc.1204240 | en_US |
dc.source.title | Oncogene | en_US |
dc.subject | DNA binding helix | en_US |
dc.subject | Hybridoma | en_US |
dc.subject | p53 | en_US |
dc.subject | Epitope | en_US |
dc.subject | Monoclonal antibody | en_US |
dc.subject | Mutant protein | en_US |
dc.subject | DNA helix | en_US |
dc.subject | DNA sequence | en_US |
dc.subject | Hybridomas | en_US |
dc.subject | Peptide Fragments | en_US |
dc.title | A monoclonal antibody against DNA binding helix of p53 protein | en_US |
dc.type | Article | en_US |
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