A monoclonal antibody against DNA binding helix of p53 protein

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dc.citation.epage1401en_US
dc.citation.issueNumber11en_US
dc.citation.spage1398en_US
dc.citation.volumeNumber20en_US
dc.contributor.authorYolcu, E.en_US
dc.contributor.authorSayan, B. S.en_US
dc.contributor.authorYağci, T.en_US
dc.contributor.authorCetin Atalay, R.en_US
dc.contributor.authorSoussi, T.en_US
dc.contributor.authorYurdusev, N.en_US
dc.contributor.authorOzturk, M.en_US
dc.date.accessioned2016-02-08T10:35:40Z
dc.date.available2016-02-08T10:35:40Z
dc.date.issued2001en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThree monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:35:40Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2001en
dc.identifier.doi10.1038/sj.onc.1204240en_US
dc.identifier.issn0950-9232
dc.identifier.urihttp://hdl.handle.net/11693/24881
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/sj.onc.1204240en_US
dc.source.titleOncogeneen_US
dc.subjectDNA binding helixen_US
dc.subjectHybridomaen_US
dc.subjectp53en_US
dc.subjectEpitopeen_US
dc.subjectMonoclonal antibodyen_US
dc.subjectMutant proteinen_US
dc.subjectDNA helixen_US
dc.subjectDNA sequenceen_US
dc.subjectHybridomasen_US
dc.subjectPeptide Fragmentsen_US
dc.titleA monoclonal antibody against DNA binding helix of p53 proteinen_US
dc.typeArticleen_US

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