Role of environmental factors in mesenchymal stem cell biology

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buir.advisorAkçalı, K. Can
dc.contributor.authorGültekin, Sinan
dc.date.accessioned2016-01-08T18:18:02Z
dc.date.available2016-01-08T18:18:02Z
dc.date.issued2009
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionAnkara : The Department of Molecular Biology and Genetics and the Institute of Engineering and Science of Bilkent University, 2009.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2009.en_US
dc.descriptionIncludes bibliographical references leaves 87-99.en_US
dc.description.abstractMesenchymal Stem Cells (MSCs) have the abilities of self-renewal and differentiation into fat, bone, cartilage, and muscle tissues. Besides intrinsic mechanisms that control the fate of the MSCs, extrinsic physiological factors also play role in this decision. Therefore, our aim is to explore the effects of possible environmental factors, involved in MSC maintenance by using rat MSCs as a model. We studied the effects of hypoxia and estrogen on growth regulation and cellular proliferation in MSCs. MSCs cells exhibited high colony number in hypoxic conditions and the expansion of MSCs was increased addition of the estrogen. In addition, estrogen prevents apoptosis, under hypoxic condition. The effects of estrogen on the expression levels of Notch genes (Notch1, Notch2, Notch3 and Notch4) were also investigated. In order to understand the possible mechanism of estrogen response, an experimental and in silico approach are used. The expression levels of Notch1 and Notch 3 were decreased treatment and the expression level of Notch 4 was increased upon estrogen treatment. In addition, bioinformatics analysis showed that, estrogen upregulates ERG family transcription factors, ELF family transcription factors, HOXL4 family transcription factors, KLF family transcription factors and transcription factor SOX3, which bind to Notch 1 transcriptional regulatory region, implying indirect effects of estrogen on Notch 1 expression. Twenty biomaterials were also investigated in order to assess whether they provide an appropriate environment for MSCs expansion. It was found that eight of the biomaterials out of twenty designated as, CA-1, CA-2, CA-3, CI-K, CI-A, CIII-1, CIII-2 and CIII-3, were appropriate candidates to expand MSCs. The combination polymers designated as HPMA/PEG provided appropriate conditions when prepared in the proportion of 1:0 (CA-1), 1:1 (CA-2) and 2:1 (CA-3). The appropriate proportion of polymers designated as HEMA/PEG/HPC was 2:1:1(CIII-1), 3:0:1 (CIII-2) and 1:1:0 (CIII-5).en_US
dc.description.degreeM.S.en_US
dc.description.provenanceMade available in DSpace on 2016-01-08T18:18:02Z (GMT). No. of bitstreams: 1 0006144.pdf: 1545000 bytes, checksum: 0fea24b0e804268b8c657699bf4ba6bf (MD5)en
dc.description.statementofresponsibilityGültekin, Sinanen_US
dc.format.extentxv, 104 leaves, illustrationsen_US
dc.identifier.urihttp://hdl.handle.net/11693/15402
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjecthypoxiaen_US
dc.subjectestrogenen_US
dc.subjectnotch receptors and biomaterialsen_US
dc.subject.lccQH588.S83 G84 2009en_US
dc.subject.lcshMesenchymal stem cells.en_US
dc.subject.lcshStem cells.en_US
dc.subject.lcshHormone therapy.en_US
dc.subject.lcshEstrogen replacement therapy.en_US
dc.titleRole of environmental factors in mesenchymal stem cell biologyen_US
dc.typeThesisen_US

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