Metastasis suppressor proteins in cutaneous squamous cell carcinoma

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dc.citation.epage615en_US
dc.citation.issueNumber7en_US
dc.citation.spage608en_US
dc.citation.volumeNumber212en_US
dc.contributor.authorBozdogan, O.en_US
dc.contributor.authorVargel, I.en_US
dc.contributor.authorCavusoglu, T.en_US
dc.contributor.authorKarabulut, A. A.en_US
dc.contributor.authorKarahan, G.en_US
dc.contributor.authorSayar, N.en_US
dc.contributor.authorAtasoy, P.en_US
dc.contributor.authorYulug, I. G.en_US
dc.date.accessioned2018-04-12T10:53:09Z
dc.date.available2018-04-12T10:53:09Z
dc.date.issued2016-07en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractCutaneous squamous cell carcinomas (cSCCs) are common human carcinomas. Despite having metastasizing capacities, they usually show less aggressive progression compared to squamous cell carcinoma (SCC) of other organs. Metastasis suppressor proteins (MSPs) are a group of proteins that control and slow-down the metastatic process. In this study, we established the importance of seven well-defined MSPs including NDRG1, NM23-H1, RhoGDI2, E-cadherin, CD82/KAI1, MKK4, and AKAP12 in cSCCs. Protein expression levels of the selected MSPs were detected in 32 cSCCs, 6 in situ SCCs, and two skin cell lines (HaCaT, A-431) by immunohistochemistry. The results were evaluated semi-quantitatively using the HSCORE system. In addition, mRNA expression levels were detected by qRT-PCR in the cell lines. The HSCOREs of NM23-H1 were similar in cSCCs and normal skin tissues, while RGHOGDI2, E-cadherin and AKAP12 were significantly downregulated in cSCCs compared to normal skin. The levels of MKK4, NDRG1 and CD82 were partially conserved in cSCCs. In stage I SCCs, nuclear staining of NM23-H1 (NM23-H1nuc) was significantly lower than in stage II/III SCCs. Only nuclear staining of MKK4 (MKK4nuc) showed significantly higher scores in in situ carcinomas compared to invasive SCCs. In conclusion, similar to other human tumors, we have demonstrated complex differential expression patterns for the MSPs in in-situ and invasive cSCCs. This complex MSP signature warrants further biological and experimental pathway research.en_US
dc.identifier.doi10.1016/j.prp.2015.12.018en_US
dc.identifier.issn0344-0338
dc.identifier.urihttp://hdl.handle.net/11693/36782
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttps://doi.org/10.1016/j.prp.2015.12.018en_US
dc.source.titlePathology Research and Practiceen_US
dc.subjectA-431en_US
dc.subjectHaCaTen_US
dc.subjectMetastasis suppressor proteinsen_US
dc.subjectSkinen_US
dc.subjectSquamous cell carcinomaen_US
dc.subjectAKAP12 proteinen_US
dc.subjectCD82 antigenen_US
dc.subjectMessenger RNAen_US
dc.subjectMetastasis suppressor proteinen_US
dc.subjectMitogen activated protein kinase kinase 4en_US
dc.subjectNDRG1 proteinen_US
dc.subjectNucleoside diphosphate kinase Aen_US
dc.subjectRhoGDI2 proteinen_US
dc.subjectTumor suppressor proteinen_US
dc.subjectUnclassified drugen_US
dc.subjectUvomorulinen_US
dc.subjectCadherinen_US
dc.subjectTumor markeren_US
dc.subjectTumor suppressor proteinen_US
dc.subjectA 431 cell lineen_US
dc.subjectCancer cell lineen_US
dc.subjectCancer stagingen_US
dc.subjectCarcinoma in situen_US
dc.subjectCell nucleusen_US
dc.subjectClinical articleen_US
dc.subjectControlled studyen_US
dc.subjectDown regulationen_US
dc.subjectFemaleen_US
dc.subjectGene expressionen_US
dc.subjectHaCaT cell lineen_US
dc.subjectHumanen_US
dc.subjectHuman cellen_US
dc.subjectHuman tissueen_US
dc.subjectImmunohistochemistryen_US
dc.subjectMaleen_US
dc.subjectProtein expressionen_US
dc.subjectReverse transcription polymerase chain reactionen_US
dc.subjectScoring systemen_US
dc.subjectSkin carcinomaen_US
dc.subjectSquamous cell carcinomaen_US
dc.subjectCarcinoma in situen_US
dc.subjectGene expression regulationen_US
dc.subjectKeratinocyteen_US
dc.subjectMetabolismen_US
dc.subjectPathologyen_US
dc.subjectSkin tumoren_US
dc.subjectSquamous cell carcinomaen_US
dc.subjectTumor cell lineen_US
dc.subjectBiomarkers, Tumoren_US
dc.subjectCadherinsen_US
dc.subjectCarcinoma in Situen_US
dc.subjectCarcinoma, Squamous Cellen_US
dc.subjectCell Line, Tumoren_US
dc.subjectGene Expression Regulation, Neoplasticen_US
dc.titleMetastasis suppressor proteins in cutaneous squamous cell carcinomaen_US
dc.typeArticleen_US

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