Characterization and corneal tissue engineering application of peptide amphiphiles

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buir.advisorTekinay, Ayşe Begüm
dc.contributor.authorDağdaş, Yavuz Selim
dc.date.accessioned2016-01-08T18:22:00Z
dc.date.available2016-01-08T18:22:00Z
dc.date.issued2012
dc.descriptionAnkara : The Materials Science and Nanotechnology Program of the Graduate School of Engineering and Science of Bilkent University, 2012.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2012.en_US
dc.descriptionIncludes bibliographical references leaves 87-92.en_US
dc.description.abstractMolecular self-assembly is a powerful technique for developing novel nanostructures by using non-covalent interactions such as hydrogen bonding, hydrophobic, electrostatic, metal-ligand, π-π and van der Waals interactions. Hydrogen bonding, hydrophobic and electrostatic interactions promote self-assembly of peptide amphiphile molecules into nanofibers. Bundles of nanofibers form a three-dimensional network resulting in gel formation. Concentration and temperature dependent measurements of gel stiffness suggest that the mechanical properties of the gels are determined by a number of factors including the interfiber interactions and mechanical properties of individual nanofibers. Peptide amphiphile molecules provide a convenient model as extracellular matrix mimetic systems for regenerative medicine studies. Since the substrate stiffness is crucial for cellular behaviours such as proliferation, adhesion and differentiation, understanding the mechanisms behind the viscoelastic properties of the gels formed by self-assembling molecules can lead to development of new materials with controlled stiffness. In this study, regeneration of the corneal stroma was used as a model system for utilization of peptide amphiphile molecules in regenerative medicine studies. Corneal stroma is constituted by collagen fiber arrays that are closely packed forming a stiff environment for corneal fibroblasts. The tunability of mechanical properties of self-assembled peptide amphiphile nanostructures was aimed to be utilized in corneal stroma regeneration. Thinning of the corneal stroma is a debilitating problem that can be caused by diseases like keratoconus, infections or accidents. Since corneal stroma has a restricted regenerative capacity, thinning of stroma is usually treated with cornea transplantation, which is limited by the number of donors. In this thesis, I studied mechanical properties of self-assembled peptide amphiphile nanostructures in nanometer and micrometer scale. I found that the divergence in gel stiffness may arise from the difference of strength of interfiber bonds. An injectable, biocompatible, biodegradable and bioactive system that can be used for thickening the corneal stroma was developed. This system that is composed of nanofibers was observed to enhance viability and proliferation of keratocytes in vitro.en_US
dc.description.provenanceMade available in DSpace on 2016-01-08T18:22:00Z (GMT). No. of bitstreams: 1 0006363.pdf: 3534165 bytes, checksum: c17d699dfdd41004cf074490a47e1dcf (MD5)en
dc.description.statementofresponsibilityDağdaş, Yavuz Selimen_US
dc.format.extentxiii, 92 leaves, illustrationsen_US
dc.identifier.urihttp://hdl.handle.net/11693/15644
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPeptide amphiphileen_US
dc.subjectself-assemblyen_US
dc.subjectnanofibersen_US
dc.subjectcross-linken_US
dc.subjectcorneal stromaen_US
dc.subjectregenerationen_US
dc.subjectrheologyen_US
dc.subjectAFMen_US
dc.subjectbiocompatibilityen_US
dc.subjectproliferationen_US
dc.subjectadhesionen_US
dc.subject.lccQP552.P4 D34 2012en_US
dc.subject.lcshPeptides.en_US
dc.subject.lcshNanostructured materials.en_US
dc.subject.lcshNanomedicine.en_US
dc.subject.lcshCornea.en_US
dc.titleCharacterization and corneal tissue engineering application of peptide amphiphilesen_US
dc.typeThesisen_US
thesis.degree.disciplineMaterials Science and Nanotechnology
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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