Saccharomyces cerevisiae C1D is implicated in both non-homologous DNA end joining and homologous recombination

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dc.citation.epage957en_US
dc.citation.issueNumber4en_US
dc.citation.spage947en_US
dc.citation.volumeNumber46en_US
dc.contributor.authorErdemir, T.en_US
dc.contributor.authorBilican, B.en_US
dc.contributor.authorCagatay, T.en_US
dc.contributor.authorGoding, C. R.en_US
dc.contributor.authorYavuzer, U.en_US
dc.date.accessioned2016-02-08T10:31:14Z
dc.date.available2016-02-08T10:31:14Z
dc.date.issued2002en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractC1D is a gamma-irradiation inducible nuclear matrix protein that interacts with and activates the DNA-dependent protein kinase (DNA-PK) that is essential for the repair of the DNA double-strand breaks and V(D)J recombination. Recently, it was demonstrated that C1D can also interact with TRAX and prevent the association of TRAX with Translin, a factor known to bind DNA break-point junctions, and that over expression of C1D can induce p53-dependent apoptosis. Taken together, these findings suggest that mammalian C1D could be involved in maintenance of genome integrity by regulating the activity of proteins involved in DNA repair and recombination. To obtain direct evidence for the biological function of C1D that we show is highly conserved between diverse species, we have analysed the Saccharomyces cerevisiae C1D homologue. We report that the disruption of the YC1D gene results in a temperature sensitivity and that yc1d mutant strains exhibit defects in non-homologous DNA end joining (NHEJ) and accurate DNA repair. In addition, using a novel plasmid-based in vivo recombination assay, we show that yc1d mutant strains are also defective in homologous recombination. These results indicate that YC1D is implicated in both homologous recombination and NHEJ pathways for the repair of DNA double-strand breaks.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:31:14Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2002en
dc.identifier.doi10.1046/j.1365-2958.2002.03224.xen_US
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/11693/24568
dc.language.isoEnglishen_US
dc.publisherBlackwell Publishing Ltden_US
dc.relation.isversionofhttp://dx.doi.org/10.1046/j.1365-2958.2002.03224.xen_US
dc.source.titleMolecular Microbiologyen_US
dc.subjectDouble stranded DNAen_US
dc.subjectFungal proteinen_US
dc.subjectNuclear proteinen_US
dc.subjectProtein C1Den_US
dc.subjectUnclassified drugen_US
dc.subjectDNA repairen_US
dc.subjectDNA strand breakageen_US
dc.titleSaccharomyces cerevisiae C1D is implicated in both non-homologous DNA end joining and homologous recombinationen_US
dc.typeArticleen_US

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