Preparation and characterization of poly(hydroxyethyl methacrylate-co-poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cells

dc.citation.epage395en_US
dc.citation.issueNumber4en_US
dc.citation.spage385en_US
dc.citation.volumeNumber19en_US
dc.contributor.authorBayramoglu, G.en_US
dc.contributor.authorAkcalı, K. C.en_US
dc.contributor.authorGultekin, S.en_US
dc.contributor.authorBengu, E.en_US
dc.contributor.authorArica, M. Y.en_US
dc.date.accessioned2019-01-25T10:57:36Z
dc.date.available2019-01-25T10:57:36Z
dc.date.issued2010-12-02en_US
dc.departmentDepartment of Chemistryen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThis study examined the effects of the surface properties of the materials, such as the hydroxyl, methyl and amino groups, on rat bone marrow derived Mesenchymal Stem Cell (MSC) seeding. A series of hydrogels were prepared in film form using 2-hydroxyethyl methacrylate (pHEMA), poly(ethyleneglycol) methacrylate (PEG-MA), and/or hydroxypropyl-chitosan (HPC). The physicochemical properties of these hydrogel films, such as water content, functional groups, contact angle, surface energy and thermal properties were affected by the composition of the materials. The ability of the MSCs to form colonies, as well as their viability on these materials were also analyzed. The water content of the hydrogel films increased with increasing PEG-MA and HPC ratio in the hydrogel. Contact angle measurements of the surface of the hydrogel films demonstrated that all the materials gave rise to a significantly hydrophilic surface compared to pure pHEMA. The blood protein interactions and platelet adhesion were reduced significantly on the surface of the materials upon the incorporation of PEG-MA compared to the control pure pHEMA and vice versa for HPC. The ability of the MSCs to adhere and form colonies on these materials was also analyzed. The results showed that these materials are suitable candidates to isolate and expand MSCs.en_US
dc.identifier.doi10.1007/s13233-011-0412-0en_US
dc.identifier.eissn2092-7673
dc.identifier.issn1598-5032
dc.identifier.urihttp://hdl.handle.net/11693/48367
dc.language.isoEnglishen_US
dc.publisherHangug Gobunja Haghoien_US
dc.relation.isversionofhttps://doi.org/10.1007/s13233-011-0412-0en_US
dc.source.titleMacromolecular Researchen_US
dc.subjectHydrogelsen_US
dc.subjectContact angleen_US
dc.subjectProtein adsorptionen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectTissue engineeringen_US
dc.titlePreparation and characterization of poly(hydroxyethyl methacrylate-co-poly(ethyleneglycol-methacrylate)/hydroxypropyl-chitosan) hydrogel films: Adhesion of rat mesenchymal stem cellsen_US
dc.typeArticleen_US
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