Nanoparticle labeling of bone marrow-derived rat mesenchymal stem cells: their use in differentiation and tracking

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dc.citation.epage9en_US
dc.citation.spage1en_US
dc.citation.volumeNumber2015en_US
dc.contributor.authorAkhan, E.en_US
dc.contributor.authorTuncel, D.en_US
dc.contributor.authorAkcali, K. C.en_US
dc.date.accessioned2016-02-08T10:03:10Z
dc.date.available2016-02-08T10:03:10Z
dc.date.issued2015en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.departmentDepartment of Chemistryen_US
dc.description.abstractMesenchymal stem cells (MSCs) are promising candidates for cellular therapies due to their ability to migrate to damaged tissue without inducing immune reaction. Many techniques have been developed to trace MSCs and their differentiation efficacy; however, all of these methods have limitations. Conjugated polymer based water-dispersible nanoparticles (CPN) represent a new class of probes because they offer high brightness, improved photostability, high fluorescent quantum yield, and noncytotoxicity comparing to conventional dyes and quantum dots. We aimed to use this tool for tracing MSCs’ fate in vitro and in vivo. MSC marker expression, survival, and differentiation capacity were assessed upon CPN treatment. Our results showed that after CPN labeling, MSC markers did not change and significant number of cells were found to be viable as revealed by MTT. Fluorescent signals were retained for 3 weeks after they were differentiated into osteocytes, adipocytes, and chondrocytes in vitro. We also showed that the labeled MSCs migrated to the site of injury and retained their labels in an in vivo liver regeneration model. The utilization of nanoparticle could be a promising tool for the tracking of MSCs in vivo and in vitro and therefore can be a useful tool to understand differentiation and homing mechanisms of MSCs.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:03:10Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2015en
dc.identifier.doi10.1155/2015/298430en_US
dc.identifier.eissn2314-6133
dc.identifier.issn2314-6141
dc.identifier.urihttp://hdl.handle.net/11693/22669
dc.language.isoEnglishen_US
dc.publisherHindawi Publishing Corporationen_US
dc.relation.isversionofhttp://dx.doi.org/10.1155/2015/298430en_US
dc.source.titleBioMed Research Internationalen_US
dc.subjectbeta1 integrinen_US
dc.titleNanoparticle labeling of bone marrow-derived rat mesenchymal stem cells: their use in differentiation and trackingen_US
dc.typeArticleen_US

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