Cancer-testis gene expression is associated with the methylenetetrahydrofolate reductase 677 C > T polymorphism in non-small cell lung carcinoma

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dc.citation.issueNumber1en_US
dc.citation.volumeNumber14en_US
dc.contributor.authorSenses, K. M.en_US
dc.contributor.authorGonen, M.en_US
dc.contributor.authorBarutcu, A. R.en_US
dc.contributor.authorKalaylioglu, Z.en_US
dc.contributor.authorIsbilen, M.en_US
dc.contributor.authorKonu, O.en_US
dc.contributor.authorChen, Y. T.en_US
dc.contributor.authorAltorki, N. K.en_US
dc.contributor.authorGure, A. O.en_US
dc.date.accessioned2016-02-08T09:35:33Z
dc.date.available2016-02-08T09:35:33Z
dc.date.issued2013en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: Tumor-specific, coordinate expression of cancer-testis (CT) genes, mapping to the X chromosome, is observed in more than 60% of non-small cell lung cancer (NSCLC) patients. Although CT gene expression has been unequivocally related to DNA demethylation of promoter regions, the underlying mechanism leading to loss of promoter methylation remains elusive. Polymorphisms of enzymes within the 1-carbon pathway have been shown to affect S-adenosyl methionine (SAM) production, which is the sole methyl donor in the cell. Allelic variants of several enzymes within this pathway have been associated with altered SAM levels either directly, or indirectly as reflected by altered levels of SAH and Homocysteine levels, and altered levels of DNA methylation. We, therefore, asked whether the five most commonly occurring polymorphisms in four of the enzymes in the 1-carbon pathway associated with CT gene expression status in patients with NSCLC.Methods: Fifty patients among a cohort of 763 with NSCLC were selected based on CT gene expression status and typed for five polymorphisms in four genes known to affect SAM generation by allele specific q-PCR and RFLP.Results: We identified a significant association between CT gene expression and the MTHFR 677 CC genotype, as well as the C allele of the SNP, in this cohort of patients. Multivariate analysis revealed that the genotype and allele strongly associate with CT gene expression, independent of potential confounders.Conclusions: Although CT gene expression is associated with DNA demethylation, in NSCLC, our data suggests this is unlikely to be the result of decreased MTHFR function. © 2013 Senses et al.; licensee BioMed Central Ltd.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:35:33Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2013en
dc.identifier.doi10.1186/1471-2350-14-97en_US
dc.identifier.issn1471-2350
dc.identifier.urihttp://hdl.handle.net/11693/20804
dc.language.isoEnglishen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/1471-2350-14-97en_US
dc.source.titleBMC Medical Geneticsen_US
dc.subjectCancer-testis genesen_US
dc.subject1-carbon pathwayen_US
dc.subjectAdometen_US
dc.subjectDNA methylationen_US
dc.titleCancer-testis gene expression is associated with the methylenetetrahydrofolate reductase 677 C > T polymorphism in non-small cell lung carcinomaen_US
dc.typeArticleen_US

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