Effects of varying levels and differing durations of calorie restriction on cellular and synaptic proteins, as well as the inflammatory state of the female mouse brain

Simple item page
buir.advisorAdams, Michelle Marie
dc.contributor.authorMacaroğlu, Duygu
dc.date.accessioned2020-10-16T08:23:08Z
dc.date.available2020-10-16T08:23:08Z
dc.date.copyright2020-09
dc.date.issued2020-09
dc.date.submitted2020-10-13
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Neuroscience, İhsan Doğramacı Bilkent University, 2020.en_US
dc.descriptionIncludes bibliographical references (leaves 74-91).en_US
dc.description.abstractAging is an inevitable and complicated process leading to functional decline. Regarding brain aging, cognitive decline takes place in multiple domains, including learning, memory, executive functions, motor coordination, and language. At the cellular and molecular level, age-related cognitive decline is elucidated with certain hallmarks, including aberrant neuronal network, stem cell exhaustion, glial cell activation, and inflammation. Calorie restriction (CR) is a widely-utilized approach for coping with aging’s detrimental effects even though there is no one agreed way for the application of CR. In this study, varying levels of CRs were applied for differing durations to MMTV-TGF-alpha female mice. The study initiated when mice were 10-weeks of age (Baseline) and carried out until 49/50 weeks of age and 81/82 weeks of age. There were four dietary groups named Adlibitum (AL; control), Chronic Calorie Restriction (CCR), Intermittent Calorie Restriction - Restriction (ICR-R), and Intermittent Calorie Restriction -Refeed (ICR-RF). The study’s first aim was to show age-related changes in the cellular and synaptic proteins and the inflammatory state of the female mice’s brains. The second aim of the study is to demonstrate the effects of varying levels of CR implemented for the short-term and the long-term manner on the same hallmarks. Our findings showed both chronic- or intermittent- CR altered the synaptic integrity proteins against brain aging at the long-term period (81/82 weeks) compared to the short-term (49/50 weeks) period except for PSD-95. Similarly, both chronic- or intermittent- CR showed an attenuative impact on the pro-inflammatory markers, but IL-6 was affected only by CCR at the same periods. Furthermore, an age-related imbalance between neurogenesis and astrogliogenesis was shown based on DCX and GFAP. Both chronic- or intermittent- CR showed a compensatory effect on it acting through astrogliogenesis, even though it was not statistically significant.en_US
dc.description.provenanceSubmitted by Betül Özen (ozen@bilkent.edu.tr) on 2020-10-16T08:23:08Z No. of bitstreams: 1 10362942.pdf: 3652619 bytes, checksum: cb8cc2d7685c1469a12f79e70798c595 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-10-16T08:23:08Z (GMT). No. of bitstreams: 1 10362942.pdf: 3652619 bytes, checksum: cb8cc2d7685c1469a12f79e70798c595 (MD5) Previous issue date: 2020-10en
dc.description.statementofresponsibilityby Duygu Macaroğluen_US
dc.embargo.release2021-04-13
dc.format.extentxvi, 91 leaves : charts (some color) ; 30 cm.en_US
dc.identifier.itemidB160696
dc.identifier.urihttp://hdl.handle.net/11693/54219
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBrain agingen_US
dc.subjectCalorie restrictionen_US
dc.subjectProliferationen_US
dc.subjectSynaptic integrityen_US
dc.subjectNeuroinflammationen_US
dc.subjectWestern bloten_US
dc.subjectFemale mouseen_US
dc.titleEffects of varying levels and differing durations of calorie restriction on cellular and synaptic proteins, as well as the inflammatory state of the female mouse brainen_US
dc.title.alternativeDeğişen düzey ve farklı sürelerdeki kalori kısıtlamasının hücresel ve sinaptik proteinler ve dişi fare beyninin inflamatuvar durumu üzerine etkilerien_US
dc.typeThesisen_US
thesis.degree.disciplineNeuroscience
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

Files

Collections

Graduate School of Engineering and Science