Pax3 and regulation of the melanocyte-specific tyrosinase-related protein-1 promoter

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dc.citation.epage26900en_US
dc.citation.issueNumber38en_US
dc.citation.spage26894en_US
dc.citation.volumeNumber274en_US
dc.contributor.authorGalibert, M. D.en_US
dc.contributor.authorYavuzer, U.en_US
dc.contributor.authorDexter, T. J.en_US
dc.contributor.authorGoding, C. R.en_US
dc.date.accessioned2016-02-08T10:40:50Z
dc.date.available2016-02-08T10:40:50Z
dc.date.issued1999en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractPrevious work has established that the melanocyte-specific tyrosinase- related protein-1 (TRP-1) promoter is regulated positively by the microphthalmia-associated transcription factor Mitf, acting through the conserved M box and negatively by the T-box factor Tbx2, which can bind two 'melanoeyte-specific elements' termed the MSEu and MSEi. Both the MSEu and MSEi, which share a 6-base pair GTGTGA consensus, are also recognized by a previously unidentified melanocyte-specific factor, MSF. Here we show using a combination of DNA binding assays, proteolytic clipping, and anti-Pax3 antibodies that MSF is indistinguishable from Pax3, a paired homeodomain transcription factor implicated genetically in melanocyte development and the regulation of the Mitf promoter. Consistent with Pax3 being able to bind the TRP-1 promoter, Pax3 is expressed in melanocytes and melanomas, and TRP-1 promoter activity is up-regulated by Pax3. The results identify a novel role for Pax3 in the expression of TRP-1, and the potential role of Pax3 in the melanoeyte lineage is discussed.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:40:50Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 1999en
dc.identifier.doi10.1074/jbc.274.38.26894en_US
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/11693/25206
dc.language.isoEnglishen_US
dc.relation.isversionofhttp://dx.doi.org/10.1074/jbc.274.38.26894en_US
dc.source.titleJournal of Biological Chemistryen_US
dc.subjectHomeodomain proteinen_US
dc.subjectMelanocyte specific factoren_US
dc.subjectTranscription factoren_US
dc.titlePax3 and regulation of the melanocyte-specific tyrosinase-related protein-1 promoteren_US
dc.typeArticleen_US

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