Stability of local brain levels of insulin-like growth factor-I in two well-characterized models of decreased plasma IGF-I

Date
2009
Advisor
Instructor
Source Title
Growth Factors
Print ISSN
0897-7194
Electronic ISSN
Publisher
Taylor & Francis
Volume
27
Issue
3
Pages
181 - 188
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Insulin-like growth factor-I (IGF-I), a functionally important neurotrophic factor, impacts tissues throughout the body including the central nervous system. In addition to the significant proportion of IGF-I that is synthesized in the liver and released into the plasma, IGF-I is expressed locally in tissues. The present study investigated the relationship between plasma and local brain levels of IGF-I in two well-characterized models of decreased IGF-I. The first is an adult-onset growth hormone deficiency (AOGHD) model, and the second is a caloric restriction (CR) model. In the first cohort of animals from both models, the hippocampus was removed from the brain immediately following decapitation, and in the second cohort, the animals were perfused transcardially with phosphate buffered saline to remove cerebral blood prior to harvesting the hippocampus. Our results demonstrated that although the plasma IGF-I levels were decreased in the CR and AOGHD rats compared to controls, the hippocampal IGF-I levels did not differ among the groups. These data suggest that local brain IGF-I levels are regulated in a different manner than plasma IGF-I levels.

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Other identifiers
Book Title
Keywords
Caloric restriction, Dwarf rat, Hippocampus, Fischer 344 × Brown Norway rat, Growth hormone
Citation
Published Version (Please cite this version)