hsa-miR-497 as a modulator of the expression in the presence or absence of chrna5 in breast cancer

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buir.advisorKonu Karakayalı, Özlen
dc.contributor.authorÖzgürsoy, Başak
dc.date.accessioned2017-07-07T10:25:37Z
dc.date.available2017-07-07T10:25:37Z
dc.date.copyright2017-06
dc.date.issued2017-06
dc.date.submitted2017-06-23
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Molecular Biology And Genetics, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves109-122).en_US
dc.description.abstractCHRNA5 is an important ligand-gated receptor with roles in addiction and in cancer. In lung cancer, CHRNA5 dysregulation is well known. There is also expression of CHRNA5 in breast cancer cell lines. microRNAs regulate mRNA expression; and different regulatory microRNAs are involved in different cancer types. microRNAs are thus potential biomarkers to diagnose the diseases (e.g. cancer). However, there is no study testing interactions between microRNAs and CHRNA5 in breast cancer. In the present study, mir-497 was found to be one of the most downregulated microRNAs with testable expression levels upon analysis of expression in the breast cancer cell line MCF7 when exposed to CHRNA5 siRNA. RT-qPCR was performed to test the expression level of mir-497. Validated target genes of mir-497 were found to be significantly related to a list of different KEGG pathways significantly (p value < 0.001) among which there were P53 and PI3K-Akt signalling pathways. Mimic-mir-497 treatment, alone or together with CHRNA5 siRNA, was applied on MCF7 cells to understand the interaction between the miRNA and siRNA under investigation. Selected target genes of mir-497 were tested; the most significantly modulated genes were involved in P53 pathway. The results indicated interactions between mir-497 and CHRNA5 however selected targets were not affected by mimicmir- 497. GSE41079 and GSE41074 public datasets containing mRNA and microRNA expression profiles of liver cancer cells treated with mimic-mir-497. Treatment were used to identify novel targets of mir-497 for future use. Immune system was detected in the second place upon REACTOME analysis of GSE41079 and GSE41074. Using multiple online microRNA-mRNA network tools mir-497 mRNA-miRNA networks were extracted for all or only immune genes. The results from network based analyses helped identify additional targets for later use in our mimic-siRNA system.en_US
dc.description.provenanceSubmitted by Betül Özen (ozen@bilkent.edu.tr) on 2017-07-07T10:25:37Z No. of bitstreams: 1 Başak ÖZGÜRSOY.pdf: 10745254 bytes, checksum: b4f8ca74df9032b16161f5f9377b6e8f (MD5)en
dc.description.provenanceMade available in DSpace on 2017-07-07T10:25:37Z (GMT). No. of bitstreams: 1 Başak ÖZGÜRSOY.pdf: 10745254 bytes, checksum: b4f8ca74df9032b16161f5f9377b6e8f (MD5) Previous issue date: 2017-06en
dc.description.statementofresponsibilityby Başak Özgürsoy.en_US
dc.embargo.release2020-06-22
dc.format.extentxx, 142 leaves : charts (some color) ; 29 cmen_US
dc.identifier.itemidB155871
dc.identifier.urihttp://hdl.handle.net/11693/33366
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMicroRNAen_US
dc.subjectBreast canceren_US
dc.subjectCHRNA5en_US
dc.subjectMir-497en_US
dc.subjectP53en_US
dc.subjectPI3K-Akten_US
dc.subjectImmune systemen_US
dc.titlehsa-miR-497 as a modulator of the expression in the presence or absence of chrna5 in breast canceren_US
dc.title.alternativemir-497 meme kanserinde, CHRNA5 ile alakalı, önemli bir regülatör mü?en_US
dc.typeThesisen_US
thesis.degree.disciplineMolecular Biology and Genetics
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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