Regulation of mineralocorticoid receptor and its downstream targets by estrogen and aldosterone in breast cancer

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buir.advisorKonu, Özlen
dc.contributor.authorÇoban, Bircan
dc.date.accessioned2016-12-09T13:51:04Z
dc.date.available2016-12-09T13:51:04Z
dc.date.copyright2016-11
dc.date.issued2016-11
dc.date.submitted2016-12-08
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (M.S.): Bilkent University, Department of Molecular Biology and Genetics, İhsan Doğramacı Bilkent University, 2016.en_US
dc.descriptionIncludes bibliographical references (leaves 107-120).en_US
dc.description.abstractMany women suffer from breast cancer worldwide thus accurate diagnosis of this disease has become an important issue for treatment options and improved clinical outcomes. Members of steroid hormone receptors, are a subfamily of nuclear receptors can serve as biomarkers in molecular classification of breast cancer. One of these, Mineralocorticoid Receptor (MR) takes part in many physiological processes in epithelial tissues including mammary epithelia, yet it is not well studied in the context of breast cancer. In this thesis, we investigated expression patterns of MR together with Glucocorticoid Receptor (GR) across multiple breast cancer cell lines at the protein level. Our study revealed that expressions of MR and GR were modulated in breast cancer as a subtype specific manner. We then enquired regulation of MR and its downstream targets, SGK1, NEDD4-2 and subunits of ENaC i.e., α, β and γ, by estrogen (E2) and aldosterone (ALDO) treatment in breast cancer via qPCR and Western Blotting. We found differential responses in expression of MR and its downstream targets to E2 and ALDO suggesting ER status was an important mediator of MR action. We also overexpressed MR in MCF7 cells and then showed that MR, NEDD4-2, β and γENaC mRNA levels increased in response to ALDO only when MR was overexpressed.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityby Bircan Çoban.en_US
dc.embargo.release2019-12-08
dc.format.extentxx, 133 leaves : charts (some color)en_US
dc.identifier.itemidB019485
dc.identifier.urihttp://hdl.handle.net/11693/32572
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiagnosisen_US
dc.subjectGRen_US
dc.subjectMR signalingen_US
dc.subjectDownstream targetsen_US
dc.subjectSGK1en_US
dc.subjectNEDD4-2en_US
dc.subjectENaC subunitsen_US
dc.subjectEstrogenen_US
dc.subjectAldosteroneen_US
dc.subjectBreast canceren_US
dc.titleRegulation of mineralocorticoid receptor and its downstream targets by estrogen and aldosterone in breast canceren_US
dc.title.alternativeMeme kanserinde mineralokortikoid reseptör ve mineralokortikoid reseptörün alt akışında yer alan hedeflerinin östrojen ve aldosteron tarafından düzenlenmesien_US
dc.typeThesisen_US

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Dept. of Molecular Biology and Genetics - Master's degree