Lithium-mediated downregulation of PKB/Akt and cyclin E with growth inhibition in hepatocellular carcinoma cells

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dc.citation.epage910en_US
dc.citation.issueNumber6en_US
dc.citation.spage903en_US
dc.citation.volumeNumber115en_US
dc.contributor.authorErdal, E.en_US
dc.contributor.authorOzturk, N.en_US
dc.contributor.authorCagatay, T.en_US
dc.contributor.authorEksioglu-Demiralp, E.en_US
dc.contributor.authorOzturk, M.en_US
dc.date.accessioned2016-02-08T10:22:50Z
dc.date.available2016-02-08T10:22:50Z
dc.date.issued2005en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractWe studied in vitro effects of glycogen synthase kinase 3β (GSK3β)-inhibitor lithium on the growth of hepatocellular carcinoma (HCC) cells. Lithium induced strong growth inhibition (>70%) in 75% (n = 9 of 12) of cell lines, apparently independent from the status of major genes that are mutated in HCC including p53, p16INK4a, β-catenin and Axin1. Comparative studies with a growth-sensitive Huh7 and growth-resistant Hep40 cell lines showed that lithium induces growth arrest in Huh7 cells but not in Hep40 cells. Lithium induced the accumulation of N-terminally phosphorylated inactive form of GSK3β with concomitant increase in β-catenin and β-catenin/TCF transcriptional activity in both cell lines. This suggests that lithium-mediated HCC growth inhibition is independent of its well-known stimulatory effect on Wnt-β-catenin signaling. The main differences between Huh7 and Hep40 responses to lithium treatment were observed at the levels PKB/Akt and cyclin E proteins. Lithium induced depletion of both proteins in growth-sensitive Huh7, but not in growth-resistant Hep40 cells. PKB/Akt and Cyclin E are 2 major proteins that are known to be constitutively active in HCC. The targeting of both proteins with lithium may be the main reason why most HCC cells are responsive to lithium-mediated growth inhibition, independent of their p53, retinoblastoma and Wnt-β-catenin pathways. The exploration of molecular mechanisms involved in lithium-mediated growth inhibition in relation with PKB/Akt and cyclin E downregulation may provide new insights for therapy of liver tumors. © 2005 Wiley-Liss, Inc.en_US
dc.identifier.doi10.1002/ijc.20972en_US
dc.identifier.issn0020-7136
dc.identifier.urihttp://hdl.handle.net/11693/24017
dc.language.isoEnglishen_US
dc.publisherWiley-Liss, Incen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/ijc.20972en_US
dc.source.titleInternational Journal of Canceren_US
dc.subjectβ-cateninen_US
dc.subjectCyclin Een_US
dc.subjectGlycogen synthase kinase-3βen_US
dc.subjectHepatomaen_US
dc.subjectLithiumen_US
dc.subjectPKB/Akten_US
dc.titleLithium-mediated downregulation of PKB/Akt and cyclin E with growth inhibition in hepatocellular carcinoma cellsen_US
dc.typeArticleen_US

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