Immunostimulatory activity of polysaccharide-poly ( I: C ) nanoparticles

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dc.citation.epage4282en_US
dc.citation.issueNumber18en_US
dc.citation.spage4275en_US
dc.citation.volumeNumber32en_US
dc.contributor.authorTincer, G.en_US
dc.contributor.authorYerlikaya, S.en_US
dc.contributor.authorYagci, F. C.en_US
dc.contributor.authorKahraman, T.en_US
dc.contributor.authorAtanur, O. M.en_US
dc.contributor.authorErbatur, O.en_US
dc.contributor.authorGursel, I.en_US
dc.date.accessioned2015-07-28T12:00:17Z
dc.date.available2015-07-28T12:00:17Z
dc.date.issued2011-06en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractImmunostimulatory properties of mushroom derived polysaccharides (PS) as stand-alone agents were tested. Next. PS were nanocomplexed with polyI:C (pIC) to yield stable nanoparticles around 200 nm in size evidenced by atomic force microscopy and dynamic light scattering analyses. PSs were selectively engaged by cells expressing TLR2 and initiated NF kappa B dependent signaling cascade leading to a Th1-biased cytokine/chemokine secretion in addition to bactericidal nitric oxide (NO) production from macrophages. Moreover, cells treated with nanoparticles led to synergistic IL6, production and upregulation of TNF alpha, MIP3 alpha, IFN gamma and IP10 transcript expression. In mice, PS-Ovalbumin-pIC formulation surpassed anti-OVA IgG responses when compared to either PS-OVA or pIC-OVA mediated immunity. Our results revealed that signal transduction initiated both by TLR2 and TLR3 via co-delivery of pIC by PS in nanoparticle depot delivery system is an effective immunization strategy. The present work implicate that the PS and nucleic acid based nanoparticle approach along with protein antigens can be harnessed to prevent infectious diseases. (C) 2011 Elsevier Ltd. All rights reserveden_US
dc.identifier.doi10.1016/j.biomaterials.2011.01.028en_US
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/11693/12153
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.biomaterials.2011.01.028en_US
dc.source.titlePolysaccharidesen_US
dc.subjectPoly(I:C)en_US
dc.subjectNanoparticlesen_US
dc.subjectTargeted delivery systemen_US
dc.subjectTLRen_US
dc.subjectImmunogenicityen_US
dc.titleImmunostimulatory activity of polysaccharide-poly ( I: C ) nanoparticlesen_US
dc.typeArticleen_US

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