Effect of double growth factor release on cartilage tissue engineering

dc.citation.epage160en_US
dc.citation.issueNumber2en_US
dc.citation.spage149en_US
dc.citation.volumeNumber7en_US
dc.contributor.authorErtan, A.B.en_US
dc.contributor.authorYilgor P.en_US
dc.contributor.authorBayyurt, B.en_US
dc.contributor.authorÇalikoǧlu, A.C.en_US
dc.contributor.authorKaspar Ç.en_US
dc.contributor.authorKök F.N.en_US
dc.contributor.authorKose G.T.en_US
dc.contributor.authorHasirci V.en_US
dc.date.accessioned2016-02-08T09:41:10Z
dc.date.available2016-02-08T09:41:10Z
dc.date.issued2013en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe effects of double release of insulin-like growth factor I (IGF-I) and growth factor β1 (TGF-β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-β1, respectively. On tissue culture polystyrene (TCPS), TGF-β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400μm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-β1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. © 2011 John Wiley & Sons, Ltd.en_US
dc.identifier.doi10.1002/term.509en_US
dc.identifier.issn19326254
dc.identifier.urihttp://hdl.handle.net/11693/21100
dc.language.isoEnglishen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/term.509en_US
dc.source.titleJournal of Tissue Engineering and Regenerative Medicineen_US
dc.subjectCartilage tissue engineeringen_US
dc.subjectCell differentiationen_US
dc.subjectGrowth factorsen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectPeptide and protein deliveryen_US
dc.subjectaggrecanen_US
dc.subjectcollagen type 2en_US
dc.subjectnanoparticleen_US
dc.subjectpoly(n isopropylacrylamide)en_US
dc.subjectpolyglactinen_US
dc.subjectpolystyreneen_US
dc.subjectsomatomedin Cen_US
dc.subjecttransforming growth factor beta1en_US
dc.subjectanimal cellen_US
dc.subjectanimal experimenten_US
dc.subjectanimal tissueen_US
dc.subjectarticleen_US
dc.subjectbone marrow cellen_US
dc.subjectcartilageen_US
dc.subjectcell differentiationen_US
dc.subjectcell growthen_US
dc.subjectcell proliferationen_US
dc.subjectcontrolled studyen_US
dc.subjectmaleen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectnonhumanen_US
dc.subjectparticle sizeen_US
dc.subjectpriority journalen_US
dc.subjectraten_US
dc.subjecttissue cultureen_US
dc.subjecttissue engineeringen_US
dc.subjectAcrylamidesen_US
dc.subjectAggrecansen_US
dc.subjectAnimalsen_US
dc.subjectCartilageen_US
dc.subjectCattleen_US
dc.subjectCell Proliferationen_US
dc.subjectCollagenen_US
dc.subjectCollagen Type IIen_US
dc.subjectExtracellular Matrixen_US
dc.subjectGlycosaminoglycansen_US
dc.subjectInsulin-Like Growth Factor Ien_US
dc.subjectLactic Aciden_US
dc.subjectMaleen_US
dc.subjectMicroscopy, Confocalen_US
dc.subjectNanoparticlesen_US
dc.subjectParticle Sizeen_US
dc.subjectPolyglycolic Aciden_US
dc.subjectPolymersen_US
dc.subjectRatsen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.subjectReal-Time Polymerase Chain Reactionen_US
dc.subjectSerum Albumin, Bovineen_US
dc.subjectTissue Engineeringen_US
dc.subjectTissue Scaffoldsen_US
dc.subjectTransforming Growth Factor beta1en_US
dc.titleEffect of double growth factor release on cartilage tissue engineeringen_US
dc.typeArticleen_US

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