Strain-and region-specific gene expression profiles in mouse brain in response to chronic nicotine treatment

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dc.citation.epage87en_US
dc.citation.issueNumber1en_US
dc.citation.spage78en_US
dc.citation.volumeNumber7en_US
dc.contributor.authorWang, J.en_US
dc.contributor.authorGutala, R.en_US
dc.contributor.authorHwang, Y. Y.en_US
dc.contributor.authorKim J. -M.en_US
dc.contributor.authorKonu, O.en_US
dc.contributor.authorMa, J. Z.en_US
dc.contributor.authorLi, M. D.en_US
dc.date.accessioned2016-02-08T10:10:24Z
dc.date.available2016-02-08T10:10:24Z
dc.date.issued2008en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractA pathway-focused complementary DNA microarray and gene ontology analysis were used to investigate gene expression profiles in the amygdala, hippocampus, nucleus accumbens, prefrontal cortex (PFC) and ventral tegmental area of C3H/HeJ and C57BL/6J mice receiving nicotine in drinking water (100 μg/ml in 2% saccharin for 2 weeks). A balanced experimental design and rigorous statistical analysis have led to the identification of 3.5-22.1% and 4.1-14.3% of the 638 sequence-verified genes as significantly modulated in the aforementioned brain regions of the C3H/HeJ and C57BL/6J strains, respectively. Comparisons of differential expression among brain tissues showed that only a small number of genes were altered in multiple brain regions, suggesting presence of a brain region-specific transcriptional response to nicotine. Subsequent principal component analysis and Expression Analysis Systematic Explorer analysis showed significant enrichment of biological processes both in C3H/HeJ and C57BL/6J mice, i.e. cell cycle/proliferation, organogenesis and transmission of nerve impulse. Finally, we verified the observed changes in expression using real-time reverse transcriptase polymerase chain reaction for six representative genes in the PFC region, providing an independent replication of our microarray results. Together, this report represents the first comprehensive gene expression profiling investigation of the changes caused by nicotine in brain tissues of the two mouse strains known to exhibit differential behavioral and physiological responses to nicotine.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:10:24Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2008en
dc.identifier.doi10.1111/j.1601-183X.2007.00328.xen_US
dc.identifier.issn1601-1848
dc.identifier.urihttp://hdl.handle.net/11693/23215
dc.language.isoEnglishen_US
dc.publisherWiley-Blackwell Publishingen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/j.1601-183X.2007.00328.xen_US
dc.source.titleGenes, Brain and Behavioren_US
dc.subjectBrain regionsen_US
dc.subjectC3H/HeJ miceen_US
dc.subjectC57BL/6J miceen_US
dc.subjectMicroarrayen_US
dc.subjectNicotineen_US
dc.subjectPCAen_US
dc.subjectDrinking wateren_US
dc.subjectSaccharinen_US
dc.subjectAmygdaloid nucleusen_US
dc.subjectAnimal experimenten_US
dc.subjectAnimal tissueen_US
dc.subjectArticleen_US
dc.subjectBehavioren_US
dc.subjectBrainen_US
dc.subjectBrain regionen_US
dc.subjectBrain tissueen_US
dc.subjectCell cycleen_US
dc.subjectCell proliferationen_US
dc.subjectControlled studyen_US
dc.subjectDNA microarrayen_US
dc.subjectGene expression profilingen_US
dc.subjectGene identificationen_US
dc.subjectHippocampusen_US
dc.subjectMouseen_US
dc.subjectNerve conductionen_US
dc.subjectNonhumanen_US
dc.subjectNucleus accumbensen_US
dc.subjectOrganogenesisen_US
dc.subjectPrefrontal cortexen_US
dc.subjectPrincipal component analysisen_US
dc.subjectPriority journalen_US
dc.subjectReal time polymerase chain reactionen_US
dc.subjectStatistical analysisen_US
dc.subjectVentral tegmentumen_US
dc.subjectAnimalsen_US
dc.subjectBehavior, animalen_US
dc.subjectCell divisionen_US
dc.subjectGene expressionen_US
dc.subjectGene expression profilingen_US
dc.subjectMaleen_US
dc.subjectMiceen_US
dc.subjectMice, Inbred C3Hen_US
dc.subjectMice, Inbred C57BLen_US
dc.subjectNeuronsen_US
dc.subjectOligonucleotide array sequence analysisen_US
dc.subjectOrganogenesisen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectReverse transcriptase polymerase chain reactionen_US
dc.subjectSpecies specificityen_US
dc.subjectSynaptic transmissionen_US
dc.subjectTranscription, geneticen_US
dc.titleStrain-and region-specific gene expression profiles in mouse brain in response to chronic nicotine treatmenten_US
dc.typeArticleen_US

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