sncRNA-1 is a small noncoding RNA produced by Mycobacterium tuberculosis in infected cells that positively regulates genes coupled to oleic acid biosynthesis

Simple item page
buir.contributor.authorBelkaya, Serkan
dc.citation.volumeNumber11en_US
dc.contributor.authorCoşkun, F. S.
dc.contributor.authorSrivastava, S.
dc.contributor.authorRaj, P.
dc.contributor.authorDozmorov, I.
dc.contributor.authorBelkaya, Serkan
dc.contributor.authorMehra, S.
dc.contributor.authorGolden, N. A.
dc.contributor.authorBucsan, A. N.
dc.contributor.authorChapagain, M. L.
dc.contributor.authorWakeland, E. K.
dc.contributor.authorKaushal, D.
dc.contributor.authorGumbo, T.
dc.contributor.authorvan Oers, N. S. C.
dc.date.accessioned2021-02-20T21:46:59Z
dc.date.available2021-02-20T21:46:59Z
dc.date.issued2020-06
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractNearly one third of the world’s population is infected with Mycobacterium tuberculosis (Mtb). While much work has focused on the role of different Mtb encoded proteins in pathogenesis, recent studies have revealed that Mtb also transcribes many noncoding RNAs whose functions remain poorly characterized. We performed RNA sequencing and identified a subset of Mtb H37Rv-encoded small RNAs (<30 nts in length) that were produced in infected macrophages. Designated as smaller noncoding RNAs (sncRNAs), three of these predominated the read counts. Each of the three, sncRNA-1, sncRNA-6, and sncRNA-8 had surrounding sequences with predicted stable secondary RNA stem loops. Site-directed mutagenesis of the precursor sequences suggest the existence of a hairpin loop dependent RNA processing mechanism. A functional assessment of sncRNA-1 suggested that it positively regulated two mycobacterial transcripts involved in oleic acid biosynthesis. Complementary loss- and gain- of-function approaches revealed that sncRNA-1 positively supports Mtb growth and survival in nutrient-depleted cultures as well as in infected macrophages. Overall, the findings reveal that Mtb produces sncRNAs in infected cells, with sncRNA-1 modulating mycobacterial gene expression including genes coupled to oleic acid biogenesis.en_US
dc.identifier.doi10.3389/fmicb.2020.01631en_US
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11693/75527
dc.language.isoEnglishen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.isversionofhttps://dx.doi.org/10.3389/fmicb.2020.01631en_US
dc.source.titleFrontiers in Microbiologyen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectSmall RNAsen_US
dc.subjectmiRNAsen_US
dc.subjectOleic aciden_US
dc.subjectGene regulationen_US
dc.titlesncRNA-1 is a small noncoding RNA produced by Mycobacterium tuberculosis in infected cells that positively regulates genes coupled to oleic acid biosynthesisen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
sncRNA-1_is_a_small_noncoding_RNA_produced_by_Mycobacterium_tuberculosis_in_infected_cells_that_positively_regulates_genes_coupled_to_oleic_acid_biosynthesis.pdf
Size:
4.11 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Department of Molecular Biology and Genetics