The Effect of estrogen on bone Marrow-Derived rat mesenchymal stem cell maintenance: inhibiting apoptosis through the expression of bcl-x l and bcl-2

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dc.citation.epage401en_US
dc.citation.issueNumber2en_US
dc.citation.spage393en_US
dc.citation.volumeNumber8en_US
dc.contributor.authorAyaloglu-Butun, F.en_US
dc.contributor.authorTerzioglu-Kara, E.en_US
dc.contributor.authorTokcaer-Keskin, Z.en_US
dc.contributor.authorAkcali, K. C.en_US
dc.date.accessioned2016-02-08T09:46:35Z
dc.date.available2016-02-08T09:46:35Z
dc.date.issued2012en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractMesenchymal Stem Cells (MSCs) have high therapeutic value for regenerative medicine and tissue engineering due to their differentiation potential and non-immunogenic characteristics. They are also considered as an effective in vivo delivery agent because of their ability to migrate to the site of injury. A major roadblock in their use for cell-based therapies is their rareness in vivo. Therefore, it is important to obtain increased number of functional MSCs in vitro in order to have adequate numbers for therapeutic regiments. We aimed to investigate the role of estrogen and its mechanism in obtaining more MSCs. MSCs were isolated from female and ovariectomized rats and cultured in the presence and absence of 10 -7 M estrogen. In the presence of estrogen, not only their CFU-F activity increased but also apoptotic rate decreased as shown by TUNEL staining leading to obtain more MSCs. Also the number of the cells in the colonies increased upon estrogen treatment. To reveal the mechanism of this effect, we focused on Bcl-2 family of proteins. Our immunoblotting experiments combined with knockdown studies suggested a critical role for anti-apoptotic Bcl-x L and Bcl-2. Estrogen treatment up regulated the expression Bcl-x L and Bcl-2. When we knocked down the expression of bcl-x L and bcl-2, MSCs lacking these genes showed an increase in the apoptotic rate in contrast to normal MSCs following estrogen treatment. Therefore, estrogen treatment will be of great advantage for cell-based therapies in order to get more functional MSCs and may provide opportunities to develop new strategies for debilitating diseases. © 2011 Springer Science+Business Media, LLC.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:46:35Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2012en
dc.identifier.doi10.1007/s12015-011-9292-0en_US
dc.identifier.issn1550-8943
dc.identifier.urihttp://hdl.handle.net/11693/21455
dc.language.isoEnglishen_US
dc.publisherSpringer Science+Business Mediaen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s12015-011-9292-0en_US
dc.source.titleStem Cell Reviews and Reportsen_US
dc.subjectApoptosisen_US
dc.subjectBcl-2en_US
dc.subjectBcl-x Len_US
dc.subjectEstrogenen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectRaten_US
dc.subjectTunelen_US
dc.titleThe Effect of estrogen on bone Marrow-Derived rat mesenchymal stem cell maintenance: inhibiting apoptosis through the expression of bcl-x l and bcl-2en_US
dc.typeArticleen_US

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