Non-intercalative, deoxyribose binding of boric acid to calf thymus DNA

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dc.citation.epage274en_US
dc.citation.issueNumber2en_US
dc.citation.spage268en_US
dc.citation.volumeNumber158en_US
dc.contributor.authorOzdemir, A.en_US
dc.contributor.authorGursacli, R. T.en_US
dc.contributor.authorTekinay, T.en_US
dc.date.accessioned2016-02-08T11:02:33Z
dc.date.available2016-02-08T11:02:33Z
dc.date.issued2014en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractThe present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K a) of 9.54x104 M -1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations. © 2014 Springer Science+Business Media.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T11:02:33Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1007/s12011-014-9924-8en_US
dc.identifier.issn0163-4984
dc.identifier.urihttp://hdl.handle.net/11693/26627
dc.language.isoEnglishen_US
dc.publisherSpringeren_US
dc.relation.isversionofhttps://doi.org/10.1007/s12011-014-9924-8en_US
dc.source.titleBiological Trace Element Researchen_US
dc.subjectBinding modeen_US
dc.subjectBoric aciden_US
dc.subjectCalf thymus DNAen_US
dc.subjectFT-IRen_US
dc.subjectSpectroscopyen_US
dc.subjectDNAen_US
dc.subjectDNAen_US
dc.subjectDNA bindingen_US
dc.subjectDNA denaturationen_US
dc.subjectDNA structureen_US
dc.subjectBovinaeen_US
dc.subjectAnimalsen_US
dc.subjectBinding Sitesen_US
dc.subjectBoric Acidsen_US
dc.subjectCalorimetryen_US
dc.subjectCattleen_US
dc.subjectDeoxyriboseen_US
dc.subjectDNAen_US
dc.titleNon-intercalative, deoxyribose binding of boric acid to calf thymus DNAen_US
dc.typeArticleen_US

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