DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D

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dc.citation.epage2199en_US
dc.citation.issueNumber14en_US
dc.citation.spage2188en_US
dc.citation.volumeNumber12en_US
dc.contributor.authorYavuzer, U.en_US
dc.contributor.authorSmith, G. C. M.en_US
dc.contributor.authorBliss, T.en_US
dc.contributor.authorWerner, D.en_US
dc.contributor.authorJackson, S. P.en_US
dc.date.accessioned2016-02-08T10:44:49Z
dc.date.available2016-02-08T10:44:49Z
dc.date.issued1998en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractDNA-dependent protein kinase (DNA-PK), which is involved in DNA double- strand break repair and V(D)J recombination, is comprised of a DNA-targeting component termed Ku and an ~465-kD catalytic subunit, DNA-PK(cs). Although DNA-PK phosphorylates proteins in the presence of DSBs or other discontinuities in the DNA double helix in vitro, the possibility exists that it is also activated in other circumstances via its association with additional proteins. Here, through use of the yeast two-hybrid screen, we discover that the recently identified high affinity DNA binding protein C1D interacts with the putative leucine zipper region of DNA-PK(cs). Furthermore, we show that C1D can interact with DNA-PK in mammalian cells and that C1D is a very effective DNA-PK substrate in vitro. Finally, we establish that C1D directs the activation of DNA-PK in a manner that does not require DNA termini. Therefore, these studies provide a function for C1D and suggest novel mechanisms for DNA-PK activation in vivo.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:44:49Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 1998en
dc.identifier.issn0890-9369
dc.identifier.urihttp://hdl.handle.net/11693/25437
dc.language.isoEnglishen_US
dc.source.titleGenes and Developmenten_US
dc.subjectC1Den_US
dc.subjectDNA repairen_US
dc.subjectDNA-PKen_US
dc.subjectNuclear matrixen_US
dc.subjectRecombinationen_US
dc.titleDNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1Den_US
dc.typeArticleen_US

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