Identification of relative protein bands in polyacrylamide gel electrophoresis (PAGE) using a multi-resolution snake algorithm
Date
1999-06
Advisor
Instructor
Source Title
BioTechniques
Print ISSN
0736-6205
Electronic ISSN
Publisher
Informa Healthcare
Volume
26
Issue
6
Pages
1162 - 1169
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract
In polyacrylamide gel electrophoresis (PAGE) image analysis, it is important to determine the percentage of the protein of interest of a protein mixture. This study presents reliable computer software to determine this percentage. The region of interest containing the protein band is detected using the snake algorithm. The iterative snake algorithm is implemented in a multi-resolutional framework. The snake is initialized on a low-resolution image. Then, the final position of the snake at the low resolution is used as the initial position in the higher-resolution image. Finally, the area of the protein is estimated as the area enclosed by the final position of the snake.
Course
Other identifiers
Book Title
Keywords
Protein, Algorithm, Analytic method, Computer program, Image analysis, Mathematical analysis, Polyacrylamide gel electrophoresis, Protein analysis, Protein determination, Electrophoresis, Polyacrylamide Gel, Software