Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders

Date
2010
Authors
Erac, Y.
Selli, C.
Kosova, B.
Akcali, K. C.
Tosun, M.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Age
Print ISSN
0161-9152
Electronic ISSN
Publisher
American Aging Association
Volume
32
Issue
2
Pages
223 - 230
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

We previously showed that the expression of transient receptor potential canonical (TRPC)6 ion channel elevated when TRPC1 was knocked down in A7r5 cultured vascular smooth muscle cells. Therefore, the purpose of this study was to explore whether TRPC6 is also upregulated in aging rat aorta comparable to that of TRPC1 in longitudinal in vivo aging model. We further investigated a possible causal relationship between altered phenylephrine-induced contractions and the expression levels of TRPC6, a purported essential component of alpha-adrenergic receptor signaling in aging aorta. Immunoblot analysis showed that TRPC1 protein levels significantly decreased whereas TRPC6 increased drastically in aorta from 16- to 20-month-old rats compared to that from 2 to 4 months. Immunohistochemical data demonstrated spatial changes in TRPC6 expression within the smooth muscle layers along with increased detection in the adventitia of the aged rat aorta. The phenylephrine-induced contractions were potentiated in aging aorta. In conclusion, based on this aging model, TRPC6 overexpression could be related with TRPC1 downregulation and might be responsible for the increased adrenoceptor sensitivity which contributes to the development of age-related vasospastic disorders. © American Aging Association 2010.

Course
Other identifiers
Book Title
Keywords
Aging, Transient receptor potential, TRPC, Vascular smooth muscle
Citation
Published Version (Please cite this version)