TIMP-2 gene transfer by positively charged PEG-lated monosized polycationic carrier to smooth muscle cells

Date
2012
Authors
Laçin, N.
Utkan, G.
Kutsal, T.
Dedeoğlu, B. G.
Yuluğ, I. G.
Pişkin, E.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Journal of Nanoparticle Research
Print ISSN
1388-0764
Electronic ISSN
1572-896X
Publisher
Springer Netherlands
Volume
14
Issue
2
Pages
1 - 9
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

Remodeling of the extracellular matrix resulting from increased secretion of metalloproteinase enzymes is implicated in restenosis following balloon angioplasty. Matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases play an essential role in both normal and pathological extracellular matrix degradation. Tissue inhibitor of matrix metalloproteinase- 2 is the most extensively studied tissue inhibitor of metalloproteinases inmyocardial tissue in animalmodels and clinical examples of cardiac disease; therefore it is selected for this study. Gene transfer of tissue inhibitor of matrix metalloproteinase-2 may have a therapeutic potential by inhibition of matrix metalloproteinase activity. We have used PEG-lated nanoparticles poly(St/PEG-EEM/DMAPM) which were synthesized previously in our laboratory. The nanoparticles, with an average size of 77.6 ± 2.05 nm with a zeta potential of +64. 4 ± 1.14 mVand 201.9 ± 1.83 nmwith +54.2 ± 0.77 mV were used in the transfection studies. Zeta Potential values and size of polyplex were appropriate for an effective transfection. TIMP-2 expression was detected by western blotting. Increased protein level in smoothmuscle cells according to non-transfected smooth muscle cells confirms the successful delivery and expression of the tissue inhibitor of matrix metalloproteinase- 2 gene with the non-viral vector transfection approach. © Springer Science+Business Media B.V. 2012.

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Other identifiers
Book Title
Keywords
Gene therapy, Nanomedicine, Nanoparticles, SMC, TIMP
Citation
Published Version (Please cite this version)