Cloning and expression profile of FLT3 gene during progenitor cell-dependent liver regeneration

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dc.citation.epage2188en_US
dc.citation.issueNumber12en_US
dc.citation.spage2181en_US
dc.citation.volumeNumber22en_US
dc.contributor.authorAydin, I. T.en_US
dc.contributor.authorTokcaer, Z.en_US
dc.contributor.authorDalgic, A.en_US
dc.contributor.authorKonu, O.en_US
dc.contributor.authorAkcali, K. C.en_US
dc.date.accessioned2016-02-08T10:15:48Z
dc.date.available2016-02-08T10:15:48Z
dc.date.issued2007en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground and Aim: The liver has a unique capacity to regenerate upon exposure to viral infections, toxic reactions and cancer formation. Liver regeneration is a complex phenomenon in which several factors participate during its onset. Cellular proliferation is an important component of this process and the factors that regulate this proliferation have a vital role. FLT3, a well-known hematopoietic stem cell and hepatic lineage surface marker, is involved in proliferative events of hematopoietic stem cells. However, its contribution to liver regeneration is not known. Therefore, the aim of this study was to clone and examine the role of FLT3 during liver regeneration in rats. Methods: Partial cDNA of rat homolog of FLT3 gene was cloned from thymus and the tissue specific expression of this gene at mRNA and protein levels was examined by RT-PCR and Western blot. After treating with 2-AAF and performing hepatectomy in rats to induce progenitor-dependent liver regeneration, the mRNA and protein expression profile of FLT3 was investigated by real-time PCR and Western blot during liver regeneration. In addition, cellular localization of FLT3 protein was determined by immunohistochemistry. Results: The results indicated that rat FLT3 cDNA has high homology with mouse and human FLT3 cDNA. It was also found that FLT3 is expressed in most of the rat tissues and during liver regeneration. In addition, its intracellular localization is altered during the late stages of liver regeneration. Conclusion: The FLT3 receptor is activated at the late stages of liver regeneration and participates in the proliferation response that is observed during progenitor-dependent liver regeneration. © 2006 The Authors.en_US
dc.identifier.doi10.1111/j.1440-1746.2006.04731.xen_US
dc.identifier.issn0815-9319
dc.identifier.urihttp://hdl.handle.net/11693/23568
dc.language.isoEnglishen_US
dc.publisherBlackwell Publishingen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/j.1440-1746.2006.04731.xen_US
dc.source.titleJournal of Gastroenterology and Hepatology (Australia)en_US
dc.subjectCloningen_US
dc.subjectFLT3en_US
dc.subjectLiver regenerationen_US
dc.subjectRaten_US
dc.subjectCD135 antigenen_US
dc.subjectComplementary DNAen_US
dc.titleCloning and expression profile of FLT3 gene during progenitor cell-dependent liver regenerationen_US
dc.typeArticleen_US

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