Regulation of Homer and group I metabotropic glutamate receptors by nicotine

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dc.citation.epage1154en_US
dc.citation.issueNumber5en_US
dc.citation.spage1145en_US
dc.citation.volumeNumber21en_US
dc.contributor.authorKane, J. K.en_US
dc.contributor.authorHwang, Y.en_US
dc.contributor.authorKonu, O.en_US
dc.contributor.authorLoughlin, S. E.en_US
dc.contributor.authorLeslie, F. M.en_US
dc.contributor.authorLi, M. D.en_US
dc.date.accessioned2016-02-08T10:24:01Z
dc.date.available2016-02-08T10:24:01Z
dc.date.issued2005en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe present study focuses on the nicotine-induced modulation of mRNA and protein expression of a number of genes involved in glutamatergic synaptic transmission in rat brain over different time periods of exposure. A subchronic (3 days) but not the chronic (7 or 14 days) administration of nicotine resulted in the up-regulation of Homer2a/b mRNA in the amygdala while in the ventral tegmental area (VTA) no change in expression of either Homer2a/b or Homer1b/c was observed. Although the increase in Homer2a/b mRNA was not translated into the protein level in the amygdala, a slight but significant up-regulation of Homer1b/c protein was observed in the same region at day 3. Both Homer forms were up-regulated at the protein level in the VTA at day 3. In the nucleus accumbens, 14 days of nicotine treatment up-regulated mRNA of Homer2b/c by 68.2% (P < 0.05), while the short form Homer1a gene was down-regulated by 65.0% at day 3 (P < 0.05). In regard to other components of the glutamatergic signalling, we identified an acute and intermittent increase in the mRNA and protein levels of mGluR1 and mGluR5 in the amygdala. In the VTA, however, the effects of nicotine on mGluR mRNA expression were long-lasting but rather specific to mGluR1. Nevertheless, mGluR1 protein levels in the VTA area were up-regulated only at day 3, as in the amygdala. These data provide further evidence for the involvement of nicotine in the glutamatergic neuronal synaptic activity in vivo, suggesting a role for the newly identified Homer proteins in this paradigm.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:24:01Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2005en
dc.identifier.doi10.1111/j.1460-9568.2005.03945.xen_US
dc.identifier.issn0953-816X
dc.identifier.urihttp://hdl.handle.net/11693/24094
dc.language.isoEnglishen_US
dc.publisherWiley-Blackwell Publishing Ltd.en_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/j.1460-9568.2005.03945.xen_US
dc.source.titleEuropean Journal of Neuroscienceen_US
dc.subjectExpressionen_US
dc.subjectHomeren_US
dc.subjectmGluRsen_US
dc.subjectPlasticityen_US
dc.subjectReal-time RT-PCRen_US
dc.subjectGene producten_US
dc.subjectMessenger RNAen_US
dc.subjectMetabotropic receptor 1en_US
dc.subjectNicotineen_US
dc.subjectProteinen_US
dc.subjectProtein Homer 1ben_US
dc.subjectProtein Homer 1cen_US
dc.subjectProtein Homer 2aen_US
dc.subjectProtein Homer 2ben_US
dc.subjectUnclassified drugen_US
dc.subjectAmygdaloid nucleusen_US
dc.subjectAnimal experimenten_US
dc.subjectAnimal modelen_US
dc.subjectArticleen_US
dc.subjectControlled studyen_US
dc.subjectGeneen_US
dc.subjectGene controlen_US
dc.subjectGene Homeren_US
dc.subjectMaleen_US
dc.subjectNonhumanen_US
dc.subjectNucleotide sequenceen_US
dc.subjectNucleus accumbensen_US
dc.subjectPriority journalen_US
dc.subjectProtein analysisen_US
dc.subjectProtein expressionen_US
dc.subjectRaten_US
dc.subjectReverse transcription polymerase chain reactionen_US
dc.subjectRNA isolationen_US
dc.subjectRNA translationen_US
dc.subjectSynaptic transmissionen_US
dc.subjectVentral tegmentumen_US
dc.subjectAmygdalaen_US
dc.subjectAnimalsen_US
dc.subjectBlotting, Westernen_US
dc.subjectCarrier Proteinsen_US
dc.subjectDrug Administration Scheduleen_US
dc.subjectGene Expressionen_US
dc.subjectGene Expression Regulationen_US
dc.subjectMaleen_US
dc.subjectModels, Neurologicalen_US
dc.subjectNicotineen_US
dc.subjectNicotinic Agonistsen_US
dc.subjectRatsen_US
dc.subjectReceptors, Metabotropic Glutamateen_US
dc.subjectReverse Transcriptase Polymerase Chain Reactionen_US
dc.subjectRNA, Messengeren_US
dc.subjectTime Factorsen_US
dc.subjectVentral Tegmental Areaen_US
dc.titleRegulation of Homer and group I metabotropic glutamate receptors by nicotineen_US
dc.typeArticleen_US

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