A synthetic biology approach for nanomaterial design, synthesis and functionalization

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buir.advisorŞeker, Urartu Özgür Şafak
dc.contributor.authorÖlmez, Tolga Tarkan
dc.date.accessioned2017-11-27T11:01:21Z
dc.date.available2017-11-27T11:01:21Z
dc.date.copyright2017-11
dc.date.issued2017-11
dc.date.submitted2017-11-24
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.descriptionThesis (Ph.D.): Bilkent University, Department of Materials Science and Nanotechnology, İhsan Doğramacı Bilkent University, 2017.en_US
dc.descriptionIncludes bibliographical references (leaves 173-191).en_US
dc.description.abstractBiological formation of inorganic material occurs in most organisms in nature. Various biomolecules such as polypeptides, lipids and metabolites are responsible for biomineralization in cells and tissues. Biological synthesis of biohybrid materials is a recently emerged discipline that uses these biomolecules in synthetic biological systems. Synthetic biology is one of most promising approaches for the development of biohybrid systems, and stands at the intersection of computer science, engineering and molecular genetics. Synthetic biology tools allow the design of programmable genetic toolkits that can compete with natural biosynthesis systems. The present thesis elaborates on the formation of well-controlled genetic systems that can synthesize and functionalize biological materials. Artificial peptides were fused to various genes through molecular genetics techniques, allowing the production of designer proteins. One aspect concerns the fusion of the 19 amino acid-long R5 motif of silaffin protein to three distinct fluorescent proteins. The R5 peptide motif can nucleate silica precursor ions to synthesize silica nanostructures. Therefore, fusion of fluorescent proteins with the R5 motif allows the synthesis and encapsulation of fluorescent silica nanoparticles. Due to its affinity to silica, R5 tag was also shown to be a candidate tag for silica resin-based affinity chromatography purification. Using synthetic biology tools, production of autonomously formed biotemplating platforms can be achieved. A bacterial functional amyloid fiber biosystem called curli can be utilized as a biotemplating platform for nanomaterials synthesis in this context. The major curli subunit CsgA was fused to artificial peptides that can nucleate and synthesize various nanomaterials. Inducible systems were also integrated into the genetic design system to confer temporal control over curli synthesis. These designs were improved through the incorporation of material-sensitive transcription factors and their cognate promoters for ions of cadmium, gold and iron. First, these material sensitive pairs were used in the development of microbial whole cell sensors that produce a fluorescence output upon induction by material precursor ions. Later, material-sensitive pairs were integrated into a modified curli nanofiber display biosystem to produce living autonomous whole cell nanomaterial synthesizers. These systems recognize precursor ions in the environment and synthesize modified curli nanofibers that can nuclate precursor ions to form functional nanomaterials.en_US
dc.description.degreePh.D.en_US
dc.description.statementofresponsibilityby Tolga Tarkan Ölmez.en_US
dc.embargo.release2020-11-24
dc.format.extentxviii, 228 leaves : charts (some color) ; 30 cmen_US
dc.identifier.itemidB156591
dc.identifier.urihttp://hdl.handle.net/11693/35634
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectR5 peptideen_US
dc.subjectSynthetic biologyen_US
dc.subjectCurli fibersen_US
dc.subjectNanomaterial synthesisen_US
dc.titleA synthetic biology approach for nanomaterial design, synthesis and functionalizationen_US
dc.title.alternativeNanomalzemelerin tasarımı, bireşimi ve işlevlendirilmesine bir bireşimsel biyoloji yaklaşımıen_US
dc.typeThesisen_US

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