Opposing roles of the aldo-keto reductases AKR1B1 and AKR1B10 in colorectal cancer

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dc.citation.epage578en_US
dc.citation.issueNumber6en_US
dc.citation.spage563en_US
dc.citation.volumeNumber40en_US
dc.contributor.authorTaskoparan, B.en_US
dc.contributor.authorSeza, E. G.en_US
dc.contributor.authorDemirkol, S.en_US
dc.contributor.authorTuncer, S.en_US
dc.contributor.authorStefek, M.en_US
dc.contributor.authorGure, A. O.en_US
dc.contributor.authorBanerjee, S.en_US
dc.date.accessioned2018-04-12T11:13:43Z
dc.date.available2018-04-12T11:13:43Z
dc.date.issued2017-09en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractPurpose: Aldo-keto reductases (including AKR1B1 and AKR1B10) constitute a family of oxidoreductases that have been implicated in the pathophysiology of diabetes and cancer, including colorectal cancer (CRC). Available data indicate that, despite their similarities in structure and enzymatic functions, their roles in CRC may be divergent. Here, we aimed to determine the expression and functional implications of AKR1B1 and AKR1B10 in CRC. Methods: AKR1B1 and AKR1B10 gene expression levels were analyzed using publicly available microarray data and ex vivo CRC-derived cDNA samples. Gene Set Enrichment Analysis (GSEA), The Cancer Genome Atlas (TCGA) RNA-seq data and The Cancer Proteome Atlas (TCPA) proteome data were analyzed to determine the effect of high and low AKR1B1 and AKR1B10 expression levels in CRC patients. Proliferation, cell cycle progression, cellular motility, adhesion and inflammation were determined in CRC-derived cell lines in which these genes were either exogenously overexpressed or silenced. Results: We found that the expression of AKR1B1 was unaltered, whereas that of AKR1B10 was decreased in primary CRCs. GSEA revealed that, while high AKR1B1 expression was associated with increased cell cycle progression, cellular motility and inflammation, high AKR1B10 expression was associated with a weak inflammatory phenotype. Functional studies carried out in CRC-derived cell lines confirmed these data. Microarray data analysis indicated that high expression levels of AKR1B1 and AKR1B10 were significantly associated with shorter and longer disease-free survival rates, respectively. A combined gene expression signature of AKR1B10 (low) and AKR1B1 (high) showed a better prognostic stratification of CRC patients independent of confounding factors. Conclusions: Despite their similarities, the expression levels and functions of AKR1B1 and AKR1B10 are highly divergent in CRC, and they may have prognostic implications.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:13:43Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017en
dc.identifier.doi10.1007/s13402-017-0351-7en_US
dc.identifier.issn2211-3428
dc.identifier.urihttp://hdl.handle.net/11693/37447
dc.language.isoEnglishen_US
dc.publisherSpringer Netherlandsen_US
dc.relation.isversionofhttps://doi.org/10.1007/s13402-017-0351-7en_US
dc.source.titleCellular Oncologyen_US
dc.subjectAKR1B1en_US
dc.subjectAKR1B10en_US
dc.subjectColorectal canceren_US
dc.subjectInflammationen_US
dc.subjectMotilityen_US
dc.subjectPrognosisen_US
dc.subjectAldo keto reductase AKR1B1en_US
dc.subjectAldo keto reductase AKR1B10en_US
dc.subjectImmunoglobulin enhancer binding proteinen_US
dc.subjectOxidoreductaseen_US
dc.subjectUnclassified drugen_US
dc.subjectArticleen_US
dc.subjectCancer prognosisen_US
dc.subjectCell cycle progressionen_US
dc.subjectCell motilityen_US
dc.subjectCell proliferationen_US
dc.subjectColorectal canceren_US
dc.subjectControlled studyen_US
dc.subjectDisease free survivalen_US
dc.subjectEnzyme activityen_US
dc.subjectGene expressionen_US
dc.subjectHumanen_US
dc.subjectInflammationen_US
dc.subjectPriority journalen_US
dc.subjectProtein expressionen_US
dc.titleOpposing roles of the aldo-keto reductases AKR1B1 and AKR1B10 in colorectal canceren_US
dc.typeArticleen_US

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