Multi-level regulation of even-skipped stripes by the ubiquitous factor Zelda

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buir.contributor.authorÖnal, Pınar
buir.contributor.orcidÖnal, Pınar|0000-0002-7143-3132
dc.citation.epage9en_US
dc.citation.issueNumber23
dc.citation.spage1
dc.citation.volumeNumber150
dc.contributor.authorÖnal, Pınar
dc.contributor.authorBishop, T.R.
dc.contributor.authorXu, Z.
dc.contributor.authorZheng, M.
dc.contributor.authorGunasinghe, M.
dc.contributor.authorNien, C.Y.
dc.contributor.authorSmall, S.
dc.contributor.authorDatta, R.R.
dc.date.accessioned2024-03-13T11:01:05Z
dc.date.available2024-03-13T11:01:05Z
dc.date.issued2023-11-23
dc.departmentDepartment of Molecular Biology and Genetics
dc.description.abstractThe zinc-finger protein Zelda (Zld) is a key activator of zygotic transcription in early Drosophila embryos. Here, we study Zld dependent regulation of the seven-striped pattern of the pair-rule gene even-skipped (eve). Individual stripes are regulated by discrete enhancers that respond to broadly distributed activators; stripe boundaries are formed by localized repressors encoded by the gap genes. The strongest effects of Zld are on stripes 2, 3 and 7, which are regulated by two enhancers in a 3.8 kb genomic fragment that includes the eve basal promoter. We show that Zld facilitates binding of the activator Bicoid and the gap repressors to this fragment, consistent with its proposed role as a pioneer protein. To test whether the effects of Zld are direct, we mutated all canonical Zld sites in the 3.8 kb fragment, which reduced expression but failed to phenocopy the abolishment of stripes caused by removing Zld in trans. We show that Zld also indirectly regulates the eve stripes by establishing specific gap gene expression boundaries, which provides the embryonic spacing required for proper stripe activation
dc.embargo.release2024-11-23
dc.identifier.doi10.1242/dev.201860
dc.identifier.eissn1477-9129
dc.identifier.issn0950-1991
dc.identifier.urihttps://hdl.handle.net/11693/114677
dc.language.isoen_US
dc.publisherThe Company of Biologists
dc.relation.isversionofhttps://doi.org/10.1242/dev.201860
dc.source.titleDevelopment
dc.subjectCis regulation
dc.subjectCross-repression
dc.subjectEmbryogenesis
dc.subjectEven-skipped
dc.subjectTranscriptional regulation
dc.subjectZelda
dc.subjectDrosophila
dc.titleMulti-level regulation of even-skipped stripes by the ubiquitous factor Zelda
dc.typeArticle

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