Molecular characterization of a full genome Turkish hepatitis C virus 1b isolate (HCV-TR1): A predominant viral form in Turkey

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dc.citation.epage177en_US
dc.citation.issueNumber2en_US
dc.citation.spage169en_US
dc.citation.volumeNumber25en_US
dc.contributor.authorYıldız, E.en_US
dc.contributor.authorOztan, A.en_US
dc.contributor.authorSar, F.en_US
dc.contributor.authorPınarbası, E.en_US
dc.contributor.authorCetin Atalay, R.en_US
dc.contributor.authorAkkız, H.en_US
dc.contributor.authorOzturk, M.en_US
dc.date.accessioned2016-02-08T10:32:10Z
dc.date.available2016-02-08T10:32:10Z
dc.date.issued2002en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBased on direct sequencing information from 5′UTR and NS5B regions, we identified subtype 1b as a predominant hepatitis C virus genome in Turkey, which affected more than 91% of 79 patients studied. Next, the full genome sequence of a Turkish 1b isolate was obtained by the cloning of polypeptide-encoding region into 7 overlapping fragments. Turkish 1b isolate, which was named HCV-TR1, comprises 9361 nucleotides, including 306 nucleotides of 5′UTR, a single long open reading frame of 9033 nucleotides, and 22 nucleotides of 3′UTR. When compared to HCV 1b polypeptide sequences available at GenBank, the predicted polypeptide displayed a total of 36 amino acid substitutions, of which 16 was specific for HCV-TR1 isolate. Despite these changes, major structural and functional motifs of HCV proteins were maintained in HCV-TR1. In contrast, HCV-TR1 displayed amino acid substitutions in 6 out of 9 major cytotoxic T-cell epitopes. These data suggest that HCV-TR1 encodes functionally intact viral proteins, but it also encodes altered viral epitopes, which may affect host immune-response.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:32:10Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2002en
dc.identifier.doi10.1023/A:1020161818764en_US
dc.identifier.issn0920-8569
dc.identifier.urihttp://hdl.handle.net/11693/24633
dc.language.isoEnglishen_US
dc.publisherKluwer Academic Publishersen_US
dc.relation.isversionofhttp://dx.doi.org/10.1023/A:1020161818764en_US
dc.source.titleVirus Genesen_US
dc.subjectHCV-TR1en_US
dc.subjectHepatitis C virusen_US
dc.subjectSubtype 1ben_US
dc.subjectViral epitopeen_US
dc.subjectNucleotideen_US
dc.subjectamino acid substitutionen_US
dc.titleMolecular characterization of a full genome Turkish hepatitis C virus 1b isolate (HCV-TR1): A predominant viral form in Turkeyen_US
dc.typeArticleen_US

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