Cd81 Interacts with the T Cell Receptor to Suppress Signaling

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dc.citation.issueNumber11en_US
dc.citation.volumeNumber7en_US
dc.contributor.authorCevik, S.I.en_US
dc.contributor.authorKeskin, N.en_US
dc.contributor.authorBelkaya, S.en_US
dc.contributor.authorOzlu, M.I.en_US
dc.contributor.authorDeniz, E.en_US
dc.contributor.authorTazebay, U.H.en_US
dc.contributor.authorErman, B.en_US
dc.date.accessioned2016-02-08T09:43:41Z
dc.date.available2016-02-08T09:43:41Z
dc.date.issued2012en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractCD81 (TAPA-1) is a ubiquitously expressed tetraspanin protein identified as a component of the B lymphocyte receptor (BCR) and as a receptor for the Hepatitis C Virus. In an effort to identify trans-membrane proteins that interact with the T-cell antigen receptor (TCR), we performed a membrane yeast two hybrid screen and identified CD81 as an interactor of the CD3delta subunit of the TCR. We found that in the absence of CD81, in thymocytes from knockout mice, TCR engagement resulted in stronger signals. These results were recapitulated in T cell lines that express low levels of CD81 through shRNA mediated silencing. Increased signaling did not result from alterations in the levels of TCR on the surface of T lymphocytes. Although CD81 is not essential for normal T lymphocyte development, it plays an important role in regulating TCR and possibly pre-TCR signal transduction by controlling the strength of signaling. CD81 dependent alterations in thymocyte signaling are evident in increased CD5 expression on CD81 deficient double positive (DP) thymocytes. We conclude that CD81 interacts with the T cell receptor to suppress signaling. © 2012 Cevik et al.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:43:41Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2012en
dc.identifier.doi10.1371/journal.pone.0050396en_US
dc.identifier.issn19326203
dc.identifier.urihttp://hdl.handle.net/11693/21250
dc.language.isoEnglishen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0050396en_US
dc.source.titlePLoS ONEen_US
dc.subjectCD3 antigenen_US
dc.subjectCD5 antigenen_US
dc.subjectCD81 antigenen_US
dc.subjectshort hairpin RNAen_US
dc.subjectT lymphocyte receptoren_US
dc.subjectT lymphocyte receptor delta chainen_US
dc.subjectantigen functionen_US
dc.subjectarticleen_US
dc.subjectcell maturationen_US
dc.subjectcontrolled studyen_US
dc.subjectgene rearrangementen_US
dc.subjectgene silencingen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectimmunoregulationen_US
dc.subjectintracellular signalingen_US
dc.subjectlymphocyte differentiationen_US
dc.subjectlymphocyte proliferationen_US
dc.subjectnucleotide sequenceen_US
dc.subjectprotein determinationen_US
dc.subjectprotein expressionen_US
dc.subjectprotein protein interactionen_US
dc.subjectthymocyteen_US
dc.subjecttwo hybrid systemen_US
dc.subjectAnimalsen_US
dc.subjectAntigens, CD5en_US
dc.subjectAntigens, CD81en_US
dc.subjectCell Communicationen_US
dc.subjectGene Expressionen_US
dc.subjectHEK293 Cellsen_US
dc.subjectHumansen_US
dc.subjectLymphocyte Activationen_US
dc.subjectMiceen_US
dc.subjectMice, Knockouten_US
dc.subjectPlasmidsen_US
dc.subjectProtein Bindingen_US
dc.subjectReceptors, Antigen, T-Cell, gamma-deltaen_US
dc.subjectRNA, Small Interferingen_US
dc.subjectSignal Transductionen_US
dc.subjectThymocytesen_US
dc.subjectTransfectionen_US
dc.subjectTwo-Hybrid System Techniquesen_US
dc.subjectHepatitis C virusen_US
dc.subjectMusen_US
dc.titleCd81 Interacts with the T Cell Receptor to Suppress Signalingen_US
dc.typeArticleen_US

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