Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: identification of BRI3 and HSF2 as novel targets

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dc.citation.epage1535en_US
dc.citation.issueNumber10en_US
dc.citation.spage1523en_US
dc.citation.volumeNumber22en_US
dc.contributor.authorKavak, E.en_US
dc.contributor.authorNajafov, A.en_US
dc.contributor.authorOzturk, N.en_US
dc.contributor.authorSeker, T.en_US
dc.contributor.authorCavusoglu, K.en_US
dc.contributor.authorAslan, T.en_US
dc.contributor.authorDuru, A. D.en_US
dc.contributor.authorSaygili, T.en_US
dc.contributor.authorHoxhaj, G.en_US
dc.contributor.authorHiz, M. C.en_US
dc.contributor.authorUnal, D. O.en_US
dc.contributor.authorBirgül-Iyison, N.en_US
dc.contributor.authorOzturk, M.en_US
dc.contributor.authorKoman, A.en_US
dc.date.accessioned2016-02-08T09:56:42Z
dc.date.available2016-02-08T09:56:42Z
dc.date.issued2010en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. β-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active β-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low β-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high β-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/β-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment. © 2010 Elsevier Inc.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:56:42Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2010en
dc.identifier.doi10.1016/j.cellsig.2010.05.021en_US
dc.identifier.issn0898-6568
dc.identifier.urihttp://hdl.handle.net/11693/22189
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.cellsig.2010.05.021en_US
dc.source.titleCellular Signallingen_US
dc.subjectBRI3en_US
dc.subjectHCCen_US
dc.subjectHSF2en_US
dc.subjectWnt/TCF4/β-catenin targetsen_US
dc.subjectXenograften_US
dc.titleAnalysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: identification of BRI3 and HSF2 as novel targetsen_US
dc.typeArticleen_US

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