In silico analysis of mutant p53(R249S) oncogenicity in hepatocellular carcinoma

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buir.advisorÇetin-Atalay, Rengül
dc.contributor.authorOvezmuradov, Guvanchmurad
dc.date.accessioned2016-07-01T11:10:00Z
dc.date.available2016-07-01T11:10:00Z
dc.date.issued2007
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionCataloged from PDF version of article.en_US
dc.description.abstractOncogenic properties of mutant p53 proteins still stand as an ill-known subject, and the mechanism responsible for this phenomenon remains to be uncovered. This thesis aims to uncover the effect of p53 codon R249S ((AGG→AGT, arginine to serine) mutation on the development of hepatocellular carcinoma (HCC) through high throughput transcriptomics analysis using oligonucleotide arrays. We compared the expression profiles of HepG2 cells carrying wt and mutant p53(R249S). Microarray data analysis revealed a molecular signature consisting of 84 differentially regulated genes, showing that the expression of mutant p53(R249S) in HepG2 cells resulted in a distinct expression profile. Furthermore, mapping these significant differentiallyexpressed genes to the p53 interaction network revealed a putative interaction network representing functional outcomes of p53(R249S) expression in the context of diverse molecular interactions. Our results clearly demonstrated that several Hepatocyte Nuclear Factors (HNF1A, HNF4A and HNF6) could play an essential role in mediating mutant p53 oncogenic activity in HCC, as the key molecules of the gene network.en_US
dc.description.degreeM.S.en_US
dc.description.statementofresponsibilityOvezmuradov, Guvanchmuraden_US
dc.format.extentx, 47 leaves, illustrations, graphicsen_US
dc.identifier.itemidBILKUTUPB003653
dc.identifier.urihttp://hdl.handle.net/11693/29985
dc.language.isoEnglishen_US
dc.publisherBilkent Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectp53en_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectmicroarrayen_US
dc.subjectgene networken_US
dc.subjectbioinformaticsen_US
dc.subject.lccQZ202 .O94 2007en_US
dc.subject.lcshp53 antioncogene.en_US
dc.titleIn silico analysis of mutant p53(R249S) oncogenicity in hepatocellular carcinomaen_US
dc.typeThesisen_US

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Dept. of Molecular Biology and Genetics - Master's degree