Studies on estradiol dependent transcriptional regulation of human Sodium Iodide Symporter gene in mammary glands

buir.advisorTazebay, Uygar H.
dc.contributor.authorGülbağcı, Neriman Tuba
dc.date.accessioned2016-07-01T10:56:27Z
dc.date.available2016-07-01T10:56:27Z
dc.date.issued2002
dc.descriptionCataloged from PDF version of article.en_US
dc.description.abstractSodium Iodide Symporter (NIS) is a transmembrane protein, which is expressed in thyroid, mammary gland (mg), stomach, and salivary gland. NIS’s transcriptional regulation in terms of cis-and trans-acting elements in thyroid gland is widely studied. However, despite identification of NIS and studies on its hormonal regulation in mammary gland, cis-and trans-acting elements controlling the mgNIS gene in this tissue are not identified yet. From in vivo experiments, it was learned that estrogen has an up regulatory effect on mgNIS transcriptional regulation. In this study, it was shown that in vitro, estrogen (even in pharmacological concentrations) was not able to induce mgNIS in estrogen receptor positive (ER(+)) MCF-7 breast carcinoma cells, and it had no additive effect on retinoic acid (RA) in NIS up regulation when it was administered in physiological concentrations. In ER (-) MDA-MB-231 breast cancarcinoma cells, ERα might be insufficient to induce mgNIS transcription inspite of the fact that ERα was able to transactivate ERE elements. Interestingly, our study indicates that tamoxifen antagonist of ER, together with estrogen induces mgNIS transcription in MCF-7 cell lines in the absence of RA. This study clearly shows the presence of a yet unidentified link between mgNIS regulation and estrogen responsive mechanisms. Bearing in mind that tamoxifen is a powerful substance in treatment of ER(+) breast cancers, and that radioactive iodide is used in thyroid cancer diagnosis and treatment. This weak induction of mgNIS expression in response to tamoxifen may also have interesting novel applications in fight against breast cancer.en_US
dc.description.provenanceMade available in DSpace on 2016-07-01T10:56:27Z (GMT). No. of bitstreams: 1 0002226.pdf: 977122 bytes, checksum: 551293bfd3ad5992b12ac2aa2b8e4dd4 (MD5) Previous issue date: 2002en
dc.description.statementofresponsibilityGülbağcı, Neriman Tubaen_US
dc.format.extentxiii, 64 leaves, illustrations, 30 cmen_US
dc.identifier.itemidBILKUTUPB067577
dc.identifier.urihttp://hdl.handle.net/11693/29243
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNISen_US
dc.subjectEstrogenen_US
dc.subjectER-αen_US
dc.subjectRAen_US
dc.subject.lccWP870 .G85 2002en_US
dc.subject.lcshBreast Neoplasms.en_US
dc.titleStudies on estradiol dependent transcriptional regulation of human Sodium Iodide Symporter gene in mammary glandsen_US
dc.typeThesisen_US
thesis.degree.disciplineMolecular Biology and Genetics
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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