MicroRNA expression patterns in canine mammary cancer show significant differences between metastatic and non-metastatic tumours

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dc.citation.epage17en_US
dc.citation.issueNumber728en_US
dc.citation.spage1en_US
dc.citation.volumeNumber17en_US
dc.contributor.authorBulkowska, M.en_US
dc.contributor.authorRybicka, A.en_US
dc.contributor.authorSenses, K. M.en_US
dc.contributor.authorUlewicz, K.en_US
dc.contributor.authorWitt, K.en_US
dc.contributor.authorSzymanska, J.en_US
dc.contributor.authorTaciak, B.en_US
dc.contributor.authorKlopfleisch, R.en_US
dc.contributor.authorHellmén, E.en_US
dc.contributor.authorDolka, I.en_US
dc.contributor.authorGure, A. O.en_US
dc.contributor.authorMucha, J.en_US
dc.contributor.authorMikow, M.en_US
dc.contributor.authorGizinski, S.en_US
dc.contributor.authorKrol, M.en_US
dc.date.accessioned2018-04-12T11:01:18Z
dc.date.available2018-04-12T11:01:18Z
dc.date.issued2017en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: MicroRNAs may act as oncogenes or tumour suppressor genes, which make these small molecules potential diagnostic/prognostic factors and targets for anticancer therapies. Several common oncogenic microRNAs have been found for canine mammary cancer and human breast cancer. On account of this, large-scale profiling of microRNA expression in canine mammary cancer seems to be important for both dogs and humans. Methods: Expression profiles of 317 microRNAs in 146 canine mammary tumours of different histological type, malignancy grade and clinical history (presence/absence of metastases) and in 25 control samples were evaluated. The profiling was performed using microarrays. Significance Analysis of Microarrays test was applied in the analysis of microarray data (both unsupervised and supervised data analyses were performed). Validation of the obtained results was performed using real-time qPCR. Subsequently, predicted targets for the microRNAs were searched for in miRBase. Results: Results of the unsupervised analysis indicate that the primary factor separating the samples is the metastasis status. Predicted targets for microRNAs differentially expressed in the metastatic vs. non-metastatic group are mostly engaged in cell cycle regulation, cell differentiation and DNA-damage repair. On the other hand, the supervised analysis reveals clusters of differentially expressed microRNAs unique for the tumour type, malignancy grade and metastasis factor. Conclusions: The most significant difference in microRNA expression was observed between the metastatic and non-metastatic group, which suggests a more important role of microRNAs in the metastasis process than in the malignant transformation. Moreover, the differentially expressed microRNAs constitute potential metastasis markers. However, validation of cfa-miR-144, cfa-miR-32 and cfa-miR-374a levels in blood samples did not follow changes observed in the non-metastatic and metastatic tumours.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:01:18Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017en
dc.identifier.doi10.1186/s12885-017-3751-1en_US
dc.identifier.issn1471-2407
dc.identifier.urihttp://hdl.handle.net/11693/37047
dc.language.isoEnglishen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.isversionofhttps://doi.org/10.1186/s12885-017-3751-1en_US
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.source.titleBMC Canceren_US
dc.subjectCanine mammary canceren_US
dc.subjectHuman breast canceren_US
dc.subjectMicroRNAen_US
dc.subjectcfa miR 144en_US
dc.subjectcfa miR 32en_US
dc.subjectcfa miR 374aen_US
dc.subjectMicroRNAen_US
dc.subjectUnclassified drugen_US
dc.subjectAnimal tissueen_US
dc.subjectBreast canceren_US
dc.subjectCancer gradingen_US
dc.subjectCell cycle regulationen_US
dc.subjectCell differentiationen_US
dc.subjectControlled studyen_US
dc.subjectDNA repairen_US
dc.subjectDogen_US
dc.subjectFemaleen_US
dc.subjectGene expressionen_US
dc.subjectMetastasisen_US
dc.subjectMicroarray analysisen_US
dc.subjectNonhumanen_US
dc.subjectReal time polymerase chain reactionen_US
dc.subjectTumor classificationen_US
dc.titleMicroRNA expression patterns in canine mammary cancer show significant differences between metastatic and non-metastatic tumoursen_US
dc.typeArticleen_US

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