Development of droplet based microfluidic system for agglutination assays

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buir.advisorElbüken, Çağlar
dc.contributor.authorMarçalı, Merve
dc.date.accessioned2016-04-18T12:58:18Z
dc.date.available2016-04-18T12:58:18Z
dc.date.copyright2015-10
dc.date.issued2015-10
dc.date.submitted26-10-2015
dc.descriptionCataloged from PDF version of thesis.en_US
dc.descriptionIncludes bibliographical references (leaves 98-107).en_US
dc.descriptionThesis (M.S.): Bilkent University, Graduate Program in Materials Science and Nanotechnology and the Graduate School of Engineering and Science, 2015.en_US
dc.description.abstractAgglutination reactions have been carried out for several applications such as assessment of bacterial infection, blood typing or detecting non-infectious diseases. To observe agglutination reactions, several approaches have been developed. Although microfluidic methods are more costly compared to the common methods in clinical practice, microfluidic systems provide a reliable and more controlled environment. Due to the ability of droplet based systems to manipulate minute volumes of fluids, microfluidic systems allow screening of several agglutination reactions at a single run. In comparison to standard microfluidic devices, droplet based microfluidic systems provide efficient mixing which is a crucial parameter for agglutination reactions. This study reports detection of agglutination reactions of whole blood in microdroplets using impedimetric measurement method. As a proof of concept demonstration of agglutination reaction, blood typing method was implemented in microdroplets. Using label free impedimetric measurement approach, agglutination reaction in microdroplets was monitored. Impedance monitoring was achieved using microelectrodes in the microchannel to measure the impedance signal generated by droplets in the continuous phase. Besides monitoring the reaction, an empirical approach was applied to find the lumped element model of the system. This model can be used as a guidance to design a detection system and to test the sensitivity of the detection system that is used for other type of immuno-diagnostic assays in microdroplets.en_US
dc.description.provenanceSubmitted by Şelale Korkut (selale@bilkent.edu.tr) on 2016-04-18T12:58:18Z No. of bitstreams: 1 10091423.pdf: 4380228 bytes, checksum: 700b0bc5a6372dc14e82176c782fe14a (MD5)en
dc.description.provenanceMade available in DSpace on 2016-04-18T12:58:18Z (GMT). No. of bitstreams: 1 10091423.pdf: 4380228 bytes, checksum: 700b0bc5a6372dc14e82176c782fe14a (MD5) Previous issue date: 2015-10en
dc.description.statementofresponsibilityby Merve Marçalı.en_US
dc.format.extentxvii, 107 leaves : illustrations, graphics.en_US
dc.identifier.itemidB151689
dc.identifier.urihttp://hdl.handle.net/11693/28939
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDroplet based microfluidicsen_US
dc.subjectDroplet mergingen_US
dc.subjectDroplet detectionen_US
dc.subjectImpedimetric measurementen_US
dc.subjectAgglutination assayen_US
dc.subjectEquivalent circuit modellingen_US
dc.titleDevelopment of droplet based microfluidic system for agglutination assaysen_US
dc.title.alternativeAglütinasyon tahlili için damlacık tabanlı mikroakışkan sistemi geliştirilmesien_US
dc.typeThesisen_US
thesis.degree.disciplineMaterials Science and Nanotechnology
thesis.degree.grantorBilkent University
thesis.degree.levelMaster's
thesis.degree.nameMS (Master of Science)

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