Generation of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterization

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buir.contributor.authorYaǧcı, Tamer
dc.citation.epage670en_US
dc.citation.issueNumber4en_US
dc.citation.spage667en_US
dc.citation.volumeNumber17en_US
dc.contributor.authorBüyüktanır, O.en_US
dc.contributor.authorYaǧcı, Tameren_US
dc.contributor.authorFindık, A.en_US
dc.contributor.authorYıldırım, T.en_US
dc.contributor.authorYurdusev, N.en_US
dc.date.accessioned2016-02-08T09:52:14Z
dc.date.available2016-02-08T09:52:14Z
dc.date.issued2011en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myeloma cells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to be reactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDa molecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessary to obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:52:14Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2011en
dc.identifier.issn1300-6045
dc.identifier.urihttp://hdl.handle.net/11693/21868
dc.language.isoTurkishen_US
dc.source.titleKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.subjectCell fusionen_US
dc.subjectHybridomaen_US
dc.subjectSalmonella enteritidisen_US
dc.subjectSpecific antibodyen_US
dc.subjectAnimaliaen_US
dc.subjectBacteria (microorganisms)en_US
dc.subjectMusen_US
dc.subjectSalmonella enteritidisen_US
dc.titleGeneration of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterizationen_US
dc.title.alternativeAnti-salmonella enteritidis antikorlari salgilayan fare hibridomalarinin geliştirilmesi ve ön karaken_US
dc.typeArticleen_US

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