Generation of mouse hybridomas secreting anti-salmonella enteritidis antibodies and their preliminary characterization

Abstract

BALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myeloma cells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to be reactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDa molecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessary to obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.