Asialo-GM1 and asialo-GM2 are putative adhesion molecules for Moraxella catarrhalis

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dc.citation.epage10en_US
dc.citation.issueNumber1en_US
dc.citation.spage5en_US
dc.citation.volumeNumber191en_US
dc.contributor.authorAhmed, K.en_US
dc.contributor.authorSuzuki, Y.en_US
dc.contributor.authorMiyamoto, D.en_US
dc.contributor.authorNagatake, T.en_US
dc.date.accessioned2016-02-08T10:33:10Z
dc.date.available2016-02-08T10:33:10Z
dc.date.issued2002en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractMoraxella catarrhalis is an important pathogen of respiratory and middle ear infections. We previously reported that the attachment of M. catarrhalis to pharyngeal epithelial cells is mediated by ganglioside M2 (GM2). Several sets of adhesins or receptors are involved in such attachment process. In this study, we used the same strains and similar bacterial culture conditions as those in our previous study, and demonstrated by thin layer chromatography that M. catarrhalis can also bind toasialo-GM1 (Gg4Cer) and asialo-GM2 (Gg3Cer). GalNAcβ1→4Galβ1 is a common sequence in both Gg4Cer and Gg3Cer, and in many respiratory bacteria, this sequence acts as a receptor for attachment to host cells. Treatment of human pharyngeal epithelial cells with anti-GM2 and anti-Gg4Cer antibodies significantlydecreased attachment of M. catarrhalis to these cells; however, treatment with anti-Gg3Cer antibody did not decrease M. catarrhalis attachment. Immunofluorescence microscopy revealed that human pharyngeal epithelial cells are positive for GM2 and Gg4Cer, but not for Gg3Cer. Our results indicate that Gg4Cer on human pharyngeal epithelial cells, and Gg3Cer, possibly on other cells, could serve as molecules for attachment of M. catarrhalis.en_US
dc.identifier.doi10.1007/s00430-002-0109-2en_US
dc.identifier.issn0300-8584
dc.identifier.urihttp://hdl.handle.net/11693/24706
dc.language.isoEnglishen_US
dc.publisherSpringer-Verlagen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s00430-002-0109-2en_US
dc.source.titleMedical Microbiology and Immunologyen_US
dc.subjectAnti-ganglioside antibodyen_US
dc.subjectAttachmenten_US
dc.subjectGangliosidesen_US
dc.subjectMoraxella catarrhalisen_US
dc.subjectSensitivity and Specificityen_US
dc.titleAsialo-GM1 and asialo-GM2 are putative adhesion molecules for Moraxella catarrhalisen_US
dc.typeArticleen_US

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