Effect of oxygen supply on metabolism of immobilized and suspended Escherichia coli

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dc.citation.epage702en_US
dc.citation.issueNumber6en_US
dc.citation.spage697en_US
dc.citation.volumeNumber51en_US
dc.contributor.authorInanç, E.en_US
dc.contributor.authorMiller J. E.en_US
dc.contributor.authorDiBiasio, D.en_US
dc.date.accessioned2016-02-08T10:50:17Z
dc.date.available2016-02-08T10:50:17Z
dc.date.issued1996en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe effect of reduced oxygen supply on the production of a recombinant protein (plasmid-encoded β-galactosidase) was investigated in Escherichia coli. A novel modified bubble tank reactor was used to provide a direct comparison between immobilized and suspended cells in identical environments except for the immobilization matrix. Decreased oxygen supply led to increased β-galactosidase synthesis by both immobilized and suspended cells. Immobilized cells produced similar amounts of β-galactosidase as the suspended cells. Lactose consumption and acetate production, on a per cell basis, were significantly higher in immobilized cells, suggesting that immobilized cells utilized fermentative metabolism. However, a transport analysis of the immobilized cell system showed that immobilized cells were not subject to either external or internal mass transfer gradients.The effect of reduced oxygen supply on the production of a recombinant protein (plasmid-encoded β-galactosidase) was investigated in Escherichia coli. A novel modified bubble tank reactor was used to provide a direct comparison between immobilized and suspended cells in identical environments except for the immobilization matrix. Decreased oxygen supply led to increased β-galactosidase synthesis by both immobilized and suspended cells. Immobilized cells produced similar amounts of β-galactosidase as the suspended cells. Lactose consumption and acetate production, on a per cell basis, were significantly higher in immobilized cells, suggesting that immobilized cells utilized fermentative metabolism. However, a transport analysis of the immobilized cell system showed that immobilized cells were not subject to either external or internal mass transfer gradients.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:50:17Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 1996en
dc.identifier.doi10.1002/(SICI)1097-0290(19960920)51:6<697en_US
dc.identifier.issn0006-3592
dc.identifier.urihttp://hdl.handle.net/11693/25776
dc.language.isoEnglishen_US
dc.publisherJohn Wiley & Sons Inc, New York, NY, United Statesen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/(SICI)1097-0290(19960920)51:6<697en_US
dc.source.titleBiotechnology and Bioengineeringen_US
dc.subjectAcetateen_US
dc.subjectAlginate immobilizationen_US
dc.subjectOxygen transporten_US
dc.subjectCell immobilizationen_US
dc.subjectComposition effectsen_US
dc.subjectEnzymesen_US
dc.subjectMass transferen_US
dc.subjectMetabolismen_US
dc.subjectOxygen supplyen_US
dc.subjectProteinsen_US
dc.titleEffect of oxygen supply on metabolism of immobilized and suspended Escherichia colien_US
dc.typeArticleen_US

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