Inflammasome induction and immunostimulatory effects of CpG-ODN loaded liposomes containing DC-cholesterol

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dc.citation.epage28en_US
dc.citation.issueNumber2en_US
dc.citation.spage21en_US
dc.citation.volumeNumber2en_US
dc.contributor.authorBayyurt, B.en_US
dc.contributor.authorGürsel I.en_US
dc.date.accessioned2016-02-08T09:42:09Z
dc.date.available2016-02-08T09:42:09Z
dc.date.issued2013en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractObjectives: This study aims to investigate the effects of cholesterol content and cationic property of liposomes on immune response. Materials and methods: Liposomes containing high amounts of 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol hydrochloride (DC-cholesterol) were prepared and loaded with K- and D-type CpG oligonucleotide (CpG-ODN) via dehydration-rehydration (DRV) method. After splenocytes and peritoneal exudate cells (PECs) primed with lipopolysaccharide (LPS) was incubated either with free or liposomal CpG-ODN counterparts, supernatants were collected and used in cytokine (IFN-g, IL-1γ and IL-1β) ELISA. Additionally, supernatants of PECs primed with LPS and stimulated with liposomes containing different doses of DC-cholesterol were collected and used in IL-1β ELISA assay. Results: Low-dose CpG-ODN loaded liposomal formulations induced higher immune activation than free CpG-ODN at the same dose. While high-dose liposomal CpG-ODN formulations decreased pro-inflammatory cytokine production in splenocytes, they increased the secretion of IL-1β. Inflammasome activation was increased in a dose dependent manner when PECs primed with LPS were incubated with only liposomes. Varying lipid molar ratios of DC-Cholesterol containing liposomes increased IL-1β production based on increasing lipid molar ratio. Conclusion: This study revealed that type and lipid ratio of liposomes may alter the cellular efficacy of the loaded immune-stimulatory agent and may initiate inflammasome activation. © 2014 Turkish Journal of Immunology.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T09:42:09Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2013en
dc.identifier.doi10.5606/tji.2014.324en_US
dc.identifier.issn1301109X
dc.identifier.urihttp://hdl.handle.net/11693/21160
dc.language.isoTurkishen_US
dc.publisherTurkish Society of Immunologyen_US
dc.relation.isversionofhttp://dx.doi.org/10.5606/tji.2014.324en_US
dc.source.titleTurkish Journal of Immunologyen_US
dc.subjectCpG-ODNen_US
dc.subjectDC-cholesterolen_US
dc.subjectImmune responseen_US
dc.subjectInflammasomeen_US
dc.subjectLiposomeen_US
dc.subject3 beta [n [ n',n' dimethylaminoethane] caramoyl]cholesterol hydrochlorideen_US
dc.subjectcationic liposomeen_US
dc.subjectCpG oligodeoxynucleotideen_US
dc.subjectgamma interferonen_US
dc.subjectinflammasomeen_US
dc.subjectinterleukin 12en_US
dc.subjectinterleukin 1betaen_US
dc.subjectlipopolysaccharideen_US
dc.subjectliposomeen_US
dc.subjectunclassified drugen_US
dc.subjectArticleen_US
dc.subjectcholesterol synthesisen_US
dc.subjectcytokine releaseen_US
dc.subjectdehydrationen_US
dc.subjectenzyme linked immunosorbent assayen_US
dc.subjecthumanen_US
dc.subjectimmune responseen_US
dc.subjectimmunostimulationen_US
dc.subjectperitoneal exudate cellen_US
dc.subjectrehydrationen_US
dc.subjectspleen cellen_US
dc.subjectsupernatanten_US
dc.titleInflammasome induction and immunostimulatory effects of CpG-ODN loaded liposomes containing DC-cholesterolen_US
dc.title.alternativeCpG-ODN yüklü DC-kolesterol içeren nano-lipozomlarin inflamazomu tetiklemeleri ve immün uyarici etkilerien_US
dc.typeArticleen_US

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Inflammasome induction and immunostimulatory effects of CpG-ODN loaded liposomes containing DC-cholesterol [CpG-ODN yüklü DC-kolesterol içeren nano-lipozomlarin inflamazomu tetiklemeleri ve immün.pdf
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