We demonstrate burst-mode operation of a polarization-maintaining Yb-doped fiber amplifier. Groups of pulses with a temporal spacing of 10 ns and 1 kHz overall repetition rate are amplified to an average pulse energy of ∼20 μJ and total burst energy of 0:25 mJ. The pulses are externally compressed to ∼400 fs. The amplifier is synchronously pulsed-pumped to minimize amplified spontaneous emission between the bursts. We characterize the influence of pump pulse duration, pump-to-signal delay, and signal burst length.