Biomimetic self-assembled peptide nanofibers for bone regeneration

buir.advisorTekinay, Ayşe Begüm
dc.contributor.authorKocabey, Samet
dc.departmentGraduate Program in Materials Science and Nanotechnologyen_US
dc.descriptionAnkara : The Materials Science and Nanotechnology Program of the Graduate School of Engineering and Science of Bilkent University, 2012.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2012.en_US
dc.descriptionIncludes bibliographical references leaves 89-97.en_US
dc.description.abstractSelf-assembled peptide nanofibers are exploited in regenerative medicine applications due to their versatile, biofunctional and extracellular-matrixresembling structures. These properties provide peptide nanofibers with osteoinductive and osteoconductive behaviors for bone regeneration applications through several approaches. In this thesis, two different approaches were discussed, which were developed to induce bone regeneration and mineralization including extracellular matrix mimicking peptide nanofibers based 2-D gel formation and surface functionalization of titanium implants. For this purpose, we designed glycosaminoglycan-mimetic peptide nanofibers inspired by chemical structure of glycosaminoglycans present in the bone extracellular matrix. We demonstrated that glycosaminoglycan-mimetic peptide nanofibers interact with BMP-2, a critical growth factor for osteogenic activity. Glycosaminoglycan-mimicking ability of the peptide nanofibers and their interaction with BMP-2 promoted osteogenic activity of and mineralization by osteoblastic cells. ALP activity, Alizarin Red Staining and EDAX spectroscopy indicated efficacy of the peptide nanofibers for inducing mineralization. We also developed a hybrid osteoconductive system for titanium biomedical implants inspired by mussel adhesion mechanism in order to overcome bone tissue integration problems. For this purpose, Dopa conjugated peptide nanofiber coating was used along with bioactive peptide sequences for osteogenic activity to enhance osseointegration of titanium surface. Dopamediated immobilization of osteogenic peptide nanofibers on titanium surfaces created an osteoconductive interface between osteoblast-like cells and inhibited adhesion and viability of soft tissue forming fibroblasts compared to the uncoated titanium substrate. In summary, osteoinductive and osteoconductive self-assembled peptide nanofibers were developed to promote osteogenic activity and mineralization of osteogenic cells. These bioactive nanofibers provide a potent platform in clinical applications of bone tissue engineering.en_US
dc.description.provenanceMade available in DSpace on 2016-01-08T18:22:00Z (GMT). No. of bitstreams: 1 0006364.pdf: 3707933 bytes, checksum: b7ddf8e1df58a3b3cc45111cdcfca785 (MD5)en
dc.description.statementofresponsibilityKocabey, Sameten_US
dc.format.extentxvii, 97 leaves, illustrationsen_US
dc.publisherBilkent Universityen_US
dc.subjectPeptide Nanofibersen_US
dc.subjectFunctional Coatingsen_US
dc.subjectSurface Modificationen_US
dc.subjectBone Regenerationen_US
dc.subjectExtracellular Matrixen_US
dc.subject.lccQP552.P4 K63 2012en_US
dc.subject.lcshNanostructured materials.en_US
dc.subject.lcshBone subtitutes.en_US
dc.titleBiomimetic self-assembled peptide nanofibers for bone regenerationen_US
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