Ectopic expression of RET results in microphthalmia and tumors in the retinal pigment epithelium

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dc.citation.epage605en_US
dc.citation.issueNumber4en_US
dc.citation.spage600en_US
dc.citation.volumeNumber80en_US
dc.contributor.authorSchmidt, A.en_US
dc.contributor.authorTief, K.en_US
dc.contributor.authorYavuzer, U.en_US
dc.contributor.authorBeermann, F.en_US
dc.date.accessioned2016-02-08T10:41:54Z
dc.date.available2016-02-08T10:41:54Z
dc.date.issued1999en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractThe retinal pigment epithelium (RPE) is essential for eye development by interacting with the overlaying neuroepithelium. Regulatory sequences of the gene encoding for tyrosinase-related protein 1 (TRP-1), linked to the lacZ reporter gene, lead to strong and specific β-galactosidase expression in the RPE. We asked how the oncogene ret would affect this epithelial cell type during mouse development. We used the TRP-1 promoter to express ret in the developing RPE, and obtained transgenic mouse lines, which showed mild to severe microphthalmia. During development, the RPE changed to a stratified epithelium with reduced or absent pigmentation from E10.5 onward. In addition, proliferation of RPE cells and tumor formation were observed from E12.5 onward. These early events prevent closure of choroid fissure and lead to microphthalmia and secondary malformations after birth. We conclude that ret transgene expression in the RPE prevents normal differentiation of this epithelial layer and induces proliferation and tumor formation. The appearance of the microphthalmic phenotype underlines the requirement of a normally developed RPE for eye development.en_US
dc.identifier.doi10.1002/(SICI)1097-0215(19990209)80:4<600en_US
dc.identifier.issn207136
dc.identifier.urihttp://hdl.handle.net/11693/25269
dc.language.isoEnglishen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/(SICI)1097-0215(19990209)80:4<600en_US
dc.source.titleInternational Journal of Canceren_US
dc.subjectAnimal tissueen_US
dc.subjectCell proliferationen_US
dc.subjectEctopic tissueen_US
dc.subjectEye developmenten_US
dc.subjectEye tumoren_US
dc.subjectMicrophthalmiaen_US
dc.subjectNeuroepitheliumen_US
dc.subjectNonhumanen_US
dc.subjectPhotoreceptoren_US
dc.titleEctopic expression of RET results in microphthalmia and tumors in the retinal pigment epitheliumen_US
dc.typeArticleen_US

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