Browsing by Subject "Three dimensional images"

Now showing 1 - 2 of 2

Open Access
Microsphere-Assisted Super-Resolved Mirau Digital Holographic Microscopy for Cell Identification
(OSA - The Optical Society, 2017) Aakhte, M.; Abbasian, V.; Akhlaghi, E. A.; Moradi, A. R.; Anand, A.; Javidi, B.
In this paper, we use a glass microsphere incorporated into a digital holographic microscope to increase the effective resolution of the system, aiming at precise cell identification. A Mirau interferometric objective is employed in the experiments, which can be used for a common-path digital holographic microscopy (DHMicroscopy) arrangement. High-magnification Mirau objectives are expensive and suffer from low working distances, yet the commonly used low-magnification Mirau objectives do not have high lateral resolutions. We show that by placing a glass microsphere within the working distance of a low-magnification Mirau objective, its effective numerical aperture can be increased, leading to super-resolved three-dimensional images. The improvement in the lateral resolution depends on the size and vertical position of microsphere, and by varying these parameters, the lateral resolution and magnification may be adjusted. We used the information from the super-resolution DHMicroscopy to identify thalassemia minor red blood cells (tRBCs). Identification is done by comparing the volumetric measurements with those of healthy RBCs. Our results show that microsphere-assisted super-resolved Mirau DHMicroscopy, being common path and off-axis in nature, has the potential to serve as a benchtop device for cell identification and biomedical measurements.
Open Access
Thereconstruction quality improvement of holographic stereograms via variable size segmentation
(IEEE, 2010) Şahin, Erdem; Onural, Levent; Kang, Hoonjong
As computer generated holograms becomes more common, the fast computation of holographic interference patterns in digital environment becomes a necessity. Since the computation time of holograms via Fresnel (or Rayleigh-Sommerfeld) diffraction models makes real time applications impossible, the holographic stereograms are developed to be a solution for this problem. Holographic stereograms divide the hologram plane into segments. In phase added stereograms the coordinates of 3D source points are used while calculating the diffraction field. And that enables to calculate the diffraction field with appropriate sized FFTs. Although the phase added stereograms are advantageous in terms of computation time, the quality of the reconstructed three dimensional images may not be satisfactory. The main reason is that the diffraction field of a given point source is approximated as a pure complex sinusuoid in each segment. To increase the reconstruction quality, we propose a method that uses variable sized segments, as opposed to previously developed holographic stereograms that use fixed sized segments. While approximating the diffraction field of a point source, higher frequency regions are covered with smaller segments and lower frequency regions with larger segments. As a result of this, we keep the total number of oscillations of pure sinusoidal waves constant in each segment. The simulations that we carried out for a point source show that we are able to obtain better quality reconstruction with our method. ©2010 IEEE.