Browsing by Subject "SSH"
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Item Open Access Human computer interaction challenges in designing pandemic trace application for the effective knowledge transfer between science and society inside the quadruple helix collaboration(Springer, Cham, 2021-07-03) Gallego, A.; Gaeta, E.; Karinsalo, A.; Ollikainen, V.; Koskela, P.; Peschke, Lutz; Folkvord, F.; Kaldoudi, E.; Jämsä, T.; Lupiáñez-Villanueva, F.; Pecchia, L.; Fico, G.; Kurosu, MasaakiIn the last decade, smartphone users grown from 2.8 billion worldwide in 2018 to 3.8 billion in 2021. This fact associates with greater ease of publishing and accessing fake news. This is a particularly concerning issue in a global crisis situation such as the COVID-19 pandemic. As stated by the WHO, this is a global health crisis and the spread of fake information could have a direct impact on people’s wellbeing. Due to this situation, all systems which compose the quadruple helix (i.e., science, economy, politics and media and culture-based public) are under great pressure. On the one hand, citizens demand fast and trusted information, and on the other hand, the scientific community is pushed to publish, resulting in scientific papers published very fast and, sometimes, without adequate peer review processes, as reflected by the unprecedented number of retreats. The PandeVITA ecosystem will contribute to offering a better understanding of how societal actors’ behave, understanding their reaction to and interaction with science and health developments in the context of pandemics, with the aim to encourage citizens to contribute to scientific research with different kinds of data. This paper describes a novel approach to citizen science interventions and user engagement based on motivational theory and behavioral science, aiming to provide a set of architectural components, technologies, tools and analytics to assess citizens’ activities, system performance and stakeholders-related key performance indicators (KPIs) in an observatory fashion, allowing to investigate the motivation of the target participants, user engagement and long-term retention.Item Open Access Identification of genes induced by BRCA1 in breast cancer cells(Academic press, 2002) Atalay, A.; Crook, T.; Ozturk, M.; Yulug, I. G.Inherited mutations of the BRCA1 gene predispose to breast, ovarian, and other cancers. The role of the BRCA1 gene in the maintenance of chromosomal integrity is linked to a number of biological properties of its protein product, including transcriptional regulation. In the present study, we have used suppression subtractive hybridisation (SSH) to identify genes induced by BRCA1 by comparing control MCF7 breast carcinoma cells (driver) with MCF7 cells ectopically expressing BRCA1 (tester) and generated a forward subtracted cDNA library. We screened 500 putative positive clones from this library. Two hundred and ten of these clones were positive by differential screening with forward and reverse subtracted probes and the 65 cDNA clones which showed more than fivefold increase were selected for sequencing analysis. We clustered 46 different genes that share high homology with sequences in the GenBank/EMBL databases. Among these, 30 were genes whose function had been previously identified while the remaining 16 clones were genes with unknown functions. Of particular interest, BRCA1 gene induces the expression of genes encoding DNA repair proteins RAD21 and MSH2, ERBB2/HER2 interacting protein ERBIN, meningioma-associated protein MAC30, and a candidate ovarian tumour-suppressor OVCA1. Northern and Western blot analyses confirmed that the expression of these five genes are up-regulated following BRCA1 overexpression in MCF7 and UBR60-bcl2 cells. This is the first study reporting a set of BRCA1-induced genes in breast carcinoma cells by the SSH technique. We suggest that some known genes identified in this study may provide new insights into the tumour-suppressor function of BRCA1. © 2002 Elsevier Science (USA). All rights reserved.